| WB | 咨询技术 | Rat |
| IF | 咨询技术 | Rat |
| IHC | 咨询技术 | Rat |
| ICC | 技术咨询 | Rat |
| FCM | 咨询技术 | Rat |
| Elisa | 咨询技术 | Rat |
| Aliases | CDF, DIA, HILDA, MLPLI |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human LIF |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇假设存在的关于Rat IgG1抗体的参考文献概括(注：内容为虚构示例，仅用于演示格式)：
1. **文献名称**: "Characterization of Rat IgG1 Monoclonal Antibodies in Immunoassays"
**作者**: Smith A, et al.
**摘要**: 研究比较了Rat IgG1与其他IgG亚类在ELISA和Western Blot中的应用，证实其高特异性但低交叉反应性，适用于低背景检测场景。
2. **文献名称**: "Structural Insights into Rat IgG1 Hinge Region Flexibility"
**作者**: Tanaka K, et al.
**摘要**: 通过X射线晶体学分析Rat IgG1铰链区结构，揭示其独特的柔韧性对抗原结合的影响，为抗体工程提供理论依据。
3. **文献名称**: "Optimization of Rat IgG1 Production in Hybridoma Cell Lines"
**作者**: Johnson R, et al.
**摘要**: 提出改良杂交瘤培养技术提升Rat IgG1产量，并验证其与流式细胞术兼容性，强调培养基成分对稳定性的关键作用。
4. **文献名称**: "Cross-Species Reactivity of Rat IgG1 in Comparative Studies"
**作者**: Müller S, et al.
**摘要**: 评估Rat IgG1在小鼠及人类组织中的交叉反应性，发现其与Fc受体结合较弱，适合需减少非特异性结合的体内实验。
(注：以上文献及作者为虚构，实际引用需查询真实数据库如PubMed)
Rat IgG1 is a subclass of immunoglobulin G (IgG) antibodies commonly found in rats and widely utilized in research due to its stability and adaptability. As part of the adaptive immune system, IgG1 constitutes a significant portion of circulating antibodies in rats. Structurally, it consists of two heavy chains (γ1) and two light chains, forming a Y-shaped molecule with antigen-binding Fab regions and an Fc region that interacts with immune cells. Compared to other IgG subclasses (e.g., IgG2a, IgG2b, IgG2c), Rat IgG1 exhibits relatively weak effector functions, such as low affinity for Fc gamma receptors (FcγRs) and limited complement activation. This makes it less effective in triggering antibody-dependent cellular cytotoxicity (ADCC) or complement-dependent cytotoxicity (CDC), but advantageous in applications where minimizing background immune activation is critical.
Rat IgG1 antibodies are frequently employed in immunoassays (e.g., flow cytometry, ELISA, immunohistochemistry) as detection or blocking reagents. They also serve as popular isotype controls to distinguish specific binding from nonspecific interactions. In therapeutic development, Rat IgG1 scaffolds are sometimes engineered for chimeric or humanized antibodies due to their predictable behavior. Notably, many commercially available monoclonal antibodies targeting cell surface markers (e.g., CD3. CD28) are of the IgG1 subclass. While their limited effector functions can be a drawback in certain therapeutic contexts, this property is often mitigated by coupling with drugs, radiolabels, or adjuvants. Overall, Rat IgG1 remains a cornerstone in biomedical research, balancing specificity with functional versatility.
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