| WB | 咨询技术 | Rat |
| IF | 咨询技术 | Rat |
| IHC | 咨询技术 | Rat |
| ICC | 技术咨询 | Rat |
| FCM | 咨询技术 | Rat |
| Elisa | 咨询技术 | Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Rat |
| Immunogen | Rat IgG2c |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇与Rat IgG2c抗体相关的文献摘要概览：
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1. **文献名称**: *"Structural and functional differences between rat IgG2c and mouse IgG2a: Implications for isotype-specific antibody engineering"*
**作者**: Smith, J.R. et al.
**摘要**: 比较了大鼠IgG2c与小鼠IgG2a的Fc区结构差异，发现两者在补体激活和Fc受体结合的效率上存在显著区别，为设计针对大鼠模型的抗体治疗提供依据。
2. **文献名称**: *"Rat IgG2c as a preferred secondary antibody for flow cytometry in autoimmune disease models"*
**作者**: Lee, H. & Kim, M.
**摘要**: 验证了大鼠IgG2c抗体在小鼠自身免疫模型中的低交叉反应性，证明其优于其他IgG亚型(如IgG1)用于流式细胞术检测，减少非特异性信号干扰。
3. **文献名称**: *"IgG subclass distribution in rat immune responses: Role of IgG2c in bacterial infection clearance"*
**作者**: Anderson, P.D. et al.
**摘要**: 分析了大鼠感染模型中IgG亚类动态变化，发现IgG2c在抗细菌(如肺炎链球菌)感染中占主导，提示其Fc介导的吞噬作用在宿主防御中的关键作用。
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**注**：以上文献为示例性质，实际引用需根据具体数据库(如PubMed)检索真实发表的论文。若需实验方案，可参考抗体公司技术手册(如BioLegend或Thermo Fisher)。
Rat IgG2c is a subclass of immunoglobulin G (IgG) found in rats, part of the adaptive immune system’s humoral response. IgG antibodies are divided into subclasses (IgG1. IgG2a, IgG2b, IgG2c, etc.) based on structural differences in their heavy chains, which influence their functional properties. Rat IgG2c, encoded by the *Ighg2c* gene, shares similarities with mouse IgG2a and IgG2c in terms of effector functions, such as complement activation and Fc receptor binding. It is typically associated with Th1-type immune responses, often elicited during viral infections or in response to protein antigens, and plays a role in pathogen neutralization, opsonization, and antibody-dependent cellular cytotoxicity (ADCC).
In research, Rat IgG2c antibodies are widely used as primary or secondary reagents in immunoassays (e.g., ELISA, Western blot, flow cytometry) and in vivo models. Their specificity and low cross-reactivity with other species’ IgG make them valuable for detecting rat-derived antigens or in dual-labeling experiments with mouse antibodies. Monoclonal Rat IgG2c antibodies are particularly favored for consistency in binding affinity and reproducibility. However, cross-reactivity with mouse IgG2a/c may occur due to structural homology, necessitating careful validation in multiplex assays.
Understanding the subclass-specific traits of Rat IgG2c—such as its half-life, Fc receptor interactions, and complement activation potential—is critical for experimental design, therapeutic applications, and interpreting immunological outcomes.
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