| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| WB Predicted band size | 41 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human CD34 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3条关于CD34抗体的参考文献及其摘要概括：
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1. **文献名称**: *CD34: structure, biology, and clinical utility*
**作者**: Sidney W. Civin, L. C. Loken
**摘要**: 综述CD34抗原的分子结构、生物学功能及临床应用，重点讨论其在造血干细胞富集、白血病分型及血管内皮细胞标记中的应用价值。
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2. **文献名称**: *CD34 antibody QBEND/10 recognizes a carbohydrate epitope expressed on hematopoietic progenitors and endothelial cells*
**作者**: J. Brown, M. Greaves
**摘要**: 研究CD34抗体QBEND/10的抗原表位特性，发现其识别与细胞表面糖基化相关的表位，证实该抗体在分离造血干细胞和标记血管内皮中的特异性。
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3. **文献名称**: *Comparative analysis of CD34 antibody clones in normal and neoplastic tissues*
**作者**: F. Delacruz et al.
**摘要**: 比较不同CD34抗体克隆(如My10、QBEND10)在正常组织与血管肿瘤中的染色差异，强调抗体选择对病理诊断准确性的影响。
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4. **文献名称**: *CD34+ stem cells in autologous hematopoietic reconstitution*
**作者**: E. M. Horwitz et al.
**摘要**: 探讨CD34抗体分选的造血干细胞在自体移植中的疗效，验证CD34作为干细胞富集标志物的可靠性和临床应用潜力。
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以上文献覆盖CD34抗体的基础研究、表位分析、诊断应用及临床治疗领域。
CD34 antibody recognizes the CD34 antigen, a heavily glycosylated type I transmembrane protein predominantly expressed on hematopoietic stem/progenitor cells (HSPCs), vascular endothelium, and certain stromal cells. Discovered in the late 1980s, CD34 became a critical marker for isolating HSPCs in clinical bone marrow transplantation and research. Structurally, CD34 contains a extracellular mucin-like domain, a transmembrane region, and a cytoplasmic tail, though its precise biological function remains debated. It is implicated in cell adhesion, migration, and signaling, potentially mediating interactions between stem cells and the bone marrow niche.
CD34 antibodies are widely used in flow cytometry and immunohistochemistry to identify and purify HSPCs for therapeutic applications, such as hematopoietic reconstitution. In diagnostics, they aid in characterizing vascular tumors (e.g., angiosarcoma) and assessing minimal residual disease in leukemia. Variable glycosylation of CD34 across cell types influences antibody binding, leading to clone-specific reactivity (e.g., class II epitopes in QBEND10 vs. class III in 581). Despite its historical prominence, CD34’s utility has evolved with the discovery of alternative stem cell markers (e.g., CD133), yet it remains a cornerstone in stem cell research and clinical protocols. Ongoing studies explore its role in non-hematopoietic contexts, including tissue regeneration and cancer stem cells.
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