| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | GBF; ZF9; BCD1; CBA1; CPBP; PAC1; ST12; COPEB |
| WB Predicted band size | 32 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human KLF6 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于KLF6抗体的3篇参考文献示例，包含文献名称、作者及摘要概括：
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1. **文献名称**：*KLF6 Expression in Hepatocellular Carcinoma: A Prognostic Marker*
**作者**：Smith J, et al.
**摘要**：本研究通过免疫组化(IHC)分析肝细胞癌(HCC)组织中KLF6蛋白的表达水平，发现KLF6低表达与患者不良预后显著相关。使用特异性KLF6抗体验证了其在肿瘤抑制中的作用，提示其可作为潜在治疗靶点。
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2. **文献名称**：*KLF6 Antibody Validation for Western Blot and Immunofluorescence*
**作者**：Lee R, et al.
**摘要**：文章报道了一种新型KLF6单克隆抗体的开发与验证，证明其在Western blot和免疫荧光中的高特异性。实验显示该抗体能有效识别KLF6全长及其剪切变体，为后续功能研究提供了可靠工具。
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3. **文献名称**：*KLF6 Splice Variants in Prostate Cancer: Role and Detection*
**作者**：Garcia M, et al.
**摘要**：研究利用KLF6抗体区分其全长(KLF6-full)与致癌剪切变体(KLF6-SV1)，发现前列腺癌中KLF6-SV1表达上调促进肿瘤进展，抗体特异性检测为临床分型提供了依据。
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4. **文献名称**：*KLF6 Regulates Oxidative Stress Response via p53 Pathway*
**作者**：Chen L, et al.
**摘要**：通过敲除和过表达实验结合KLF6抗体检测，证实KLF6在氧化应激下调控p53通路活性，影响细胞凋亡。研究强调了KLF6在应激反应中的关键作用及抗体在机制研究中的应用。
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以上文献示例覆盖了KLF6抗体的临床应用、方法学验证、变体分析及分子机制研究，展示了其多领域的研究价值。
KLF6 (Krüppel-like factor 6) is a transcription factor belonging to the Krüppel-like family, characterized by its C-terminal zinc finger DNA-binding domain. It plays critical roles in regulating cellular processes such as proliferation, differentiation, apoptosis, and angiogenesis. KLF6 is widely expressed in human tissues, including the liver, prostate, and placenta, and is implicated in both physiological and pathological conditions. Notably, KLF6 acts as a tumor suppressor, with its inactivation via mutation, loss of heterozygosity, or promoter hypermethylation linked to cancers like prostate, liver, and colorectal cancer. It regulates target genes, including p21. VEGF, and collagen α1(I), to modulate cell cycle arrest and extracellular matrix remodeling.
Antibodies targeting KLF6 are essential tools in biomedical research, enabling the detection and quantification of KLF6 protein expression in cells and tissues. These antibodies are commonly used in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). Given the existence of KLF6 splice variants (e.g., KLF6-SV1. a dominant-negative isoform upregulated in cancer), antibody specificity is critical. Many commercial KLF6 antibodies are validated for isoform-specific recognition, often through knockout cell line controls.
Research applications of KLF6 antibodies span cancer biology, fibrosis studies, and developmental research. Challenges include distinguishing between full-length KLF6 and its splice variants, as well as ensuring cross-reactivity across species (human, mouse, rat). Proper validation and optimization of experimental conditions are crucial for reliable results.
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