| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | BETA3AR |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human ADRB3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于KDM5B抗体的3篇参考文献示例(内容为模拟虚构，仅供参考)：
1. **文献名称**：KDM5B Antibody Validation in Epigenetic Regulation of Breast Cancer
**作者**：Smith J, et al.
**摘要**：本研究验证了一种高特异性KDM5B抗体在乳腺癌细胞系中的应用，通过Western blot和免疫组化证实KDM5B在肿瘤组织中的高表达，并揭示其通过调控H3K4me3水平促进癌细胞增殖的机制。
2. **文献名称**：Role of KDM5B in Drug Resistance: Insights from Antibody-Based Chromatin Profiling
**作者**：Chen L, et al.
**摘要**：利用KDM5B抗体进行ChIP-seq分析，发现KDM5B在化疗耐药性卵巢癌细胞中靶向特定基因启动子区域，降低H3K4me3修饰，导致DNA修复基因沉默，从而增强耐药性。
3. **文献名称**：Development of a Monoclonal Antibody for KDM5B and Its Application in Glioblastoma Studies
**作者**：Wang Y, et al.
**摘要**：开发了一种新型KDM5B单克隆抗体，通过免疫荧光和流式细胞术验证其在胶质母细胞瘤中的亚细胞定位，证明KDM5B通过抑制分化相关基因参与肿瘤干细胞维持。
4. **文献名称**：KDM5B Antibody Reveals Epigenetic Dysregulation in Aging Neurons
**作者**：Garcia R, et al.
**摘要**：使用KDM5B抗体分析老年小鼠脑组织，发现KDM5B表达上调导致神经元H3K4me3水平下降，与认知功能衰退相关，提示其作为神经退行性疾病的潜在治疗靶点。
(注：上述文献为示例，实际引用需检索PubMed、Google Scholar等数据库获取真实信息。)
KDM5B (lysine-specific demethylase 5B), also known as JARID1B or PLU-1. is a histone-modifying enzyme belonging to the KDM5/JARID1 subfamily of lysine demethylases. It specifically removes di- and tri-methyl groups from histone H3 lysine 4 (H3K4me2/me3), a chromatin mark associated with active transcription, thereby regulating gene expression through epigenetic modulation. KDM5B plays critical roles in cell differentiation, cell cycle progression, and stem cell maintenance. Dysregulation of KDM5B has been implicated in various cancers, including breast cancer, melanoma, and lung cancer, where its overexpression is linked to tumor progression, drug resistance, and poor prognosis.
KDM5B antibodies are essential tools for studying its expression, localization, and function in both normal and disease contexts. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP-seq). Validating antibody specificity is crucial, often achieved using knockout cell lines or siRNA-mediated knockdown controls. Researchers also utilize KDM5B antibodies to explore its interaction with chromatin-modifying complexes and its role in epigenetic reprogramming.
The development of KDM5B-targeted therapies, including small-molecule inhibitors, has heightened interest in reliable detection methods. High-quality antibodies enable mechanistic studies, biomarker discovery, and preclinical evaluation of therapeutic strategies aimed at modulating KDM5B activity in cancer and other diseases.
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