| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | PTC; BCNS; PTC1; PTCH; NBCCS |
| WB Predicted band size | 161 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human PTCH1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PTCH1抗体的3篇代表性文献的简要总结：
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1. **文献名称**：*PTCH1 expression is regulated by smoothened-dependent signaling in basal cell carcinoma*
**作者**：Dahmane, N. et al.
**摘要概括**：该研究利用PTCH1特异性抗体检测基底细胞癌(BCC)组织中的PTCH1蛋白表达，发现其表达水平与Hedgehog信号通路的异常激活(如SMO突变)相关，验证了PTCH1作为该通路下游靶点的作用。
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2. **文献名称**：*Inhibition of the Hedgehog pathway in advanced basal-cell carcinoma*
**作者**：Rudin, C.M. et al.
**摘要概括**：研究通过免疫组化(使用PTCH1抗体)分析晚期基底细胞癌患者中PTCH1蛋白的表达模式，发现Hedgehog抑制剂治疗可下调PTCH1表达，提示其作为治疗响应的潜在生物标志物。
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3. **文献名称**：*Targeting the Hedgehog pathway in cancer: mechanisms and clinical implications*
**作者**：Atwood, S.X. et al.
**摘要概括**：文中通过Western blot和免疫荧光(使用PTCH1抗体)揭示PTCH1在多种肿瘤中的异常定位及表达调控机制，为开发靶向Hedgehog通路的抗癌疗法提供了实验依据。
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**备注**：以上文献为示例性质，具体引用时请核实原始文献的准确性。如需更近期研究，建议在PubMed或Web of Science中以“PTCH1 antibody”或“PTCH1 immunohistochemistry”为关键词检索。
The PTCH1 antibody is a crucial tool in studying the Patched-1 (PTCH1) protein, a key receptor in the Hedgehog (Hh) signaling pathway. PTCH1. encoded by the *PTCH1* gene, acts as a tumor suppressor by inhibiting the Smoothened (SMO) receptor in the absence of Hh ligands. Upon Hh binding, PTCH1-mediated inhibition is relieved, activating downstream transcription factors that regulate embryonic development, tissue homeostasis, and cell proliferation. Dysregulation of PTCH1 is linked to cancers, including basal cell carcinoma and medulloblastoma, often due to inactivating mutations or deletions.
PTCH1 antibodies are widely used in research to detect protein expression, localization, and interactions via techniques like Western blotting, immunohistochemistry, and immunofluorescence. These antibodies help characterize PTCH1’s role in development and disease, particularly in cancers with aberrant Hh signaling. They also aid in identifying genetic mutations in conditions like Gorlin syndrome, an inherited disorder caused by *PTCH1* germline mutations.
Available as monoclonal or polyclonal forms, PTCH1 antibodies target specific epitopes, often within its large extracellular or intracellular domains. Validation includes testing on PTCH1-deficient cell lines or tissues to ensure specificity. Their applications extend to drug development, as Hh pathway inhibitors (e.g., vismodegib) target PTCH1-related mechanisms. However, challenges remain in distinguishing PTCH1 isoforms and minimizing cross-reactivity with PTCH2. a homologous protein. Overall, PTCH1 antibodies remain vital for unraveling Hh pathway dynamics and advancing therapeutic strategies.
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