| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/40-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | 2BP1; FOX1; A2BP1; FOX-1; HRNBP1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human RBFOX1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
1. **"RBFOX1 regulates neuronal splicing during development in mammals"**
- **作者**: Conboy, J.G. et al.
- **摘要**: 本研究利用RBFOX1特异性抗体，通过免疫沉淀和Western blot技术，揭示了RBFOX1在哺乳动物神经元发育过程中调控mRNA剪接的作用，并发现其缺失与突触功能异常相关。
2. **"RBFOX1 haploinsufficiency leads to altered brain network activity and autism-associated behaviors"**
- **作者**: Gehman, L.T. et al.
- **摘要**: 通过免疫组化结合RBFOX1抗体，研究发现RBFOX1单倍剂量不足会改变小鼠脑网络活动，导致自闭症样行为，提示其在神经精神疾病中的潜在机制。
3. **"A post-transcriptional regulatory switch by RNA-binding proteins in neurodegeneration"**
- **作者**: Lee, J.A. & Zheng, Y.
- **摘要**: 该文献通过RBFOX1抗体进行蛋白质定位分析，发现其在神经退行性疾病模型中通过结合特定RNA靶点调控基因表达，影响神经元存活。
4. **"Cell-type-specific CLIP reveals differential roles of RBFOX proteins in brain development"**
- **作者**: Weyn-Vanhentenryck, S.M. et al.
- **摘要**: 利用RBFOX1抗体进行细胞特异性CLIP-seq实验，揭示了其在神经元亚型中的差异性剪接调控功能，并关联到神经发育疾病的遗传风险位点。
The RBFOX1 antibody is a crucial tool in neuroscience and molecular biology research, targeting the RNA Binding Fox-1 Homolog 1 (RBFOX1) protein, also known as A2BP1 or Fox-1. RBFOX1 is a conserved RNA-binding protein involved in alternative splicing, mRNA stability, and transcriptional regulation, primarily expressed in neurons, muscle cells, and the heart. It plays a vital role in neurodevelopment, synaptic plasticity, and neuronal excitability, with genetic variations linked to neurodevelopmental disorders (e.g., autism, epilepsy) and cardiovascular diseases.
RBFOX1 antibodies are widely used to detect protein expression, localization, and interactions in experimental models (e.g., Western blot, immunohistochemistry, immunofluorescence). They help elucidate RBFOX1's functional mechanisms in tissue-specific splicing networks and disease pathways. Commercial RBFOX1 antibodies are typically raised in hosts like rabbits or mice, targeting specific epitopes (e.g., N-terminal or C-terminal regions). Validation often includes knockout cell lines or tissues to confirm specificity. Researchers prioritize antibodies validated for cross-reactivity across species (human, mouse, rat) and applications.
Recent studies using these antibodies have uncovered RBFOX1's role in regulating autism-related genes and ion channel splicing, highlighting its therapeutic potential. However, variability in antibody performance (e.g., batch differences, non-specific binding) requires careful optimization in experimental setups. Proper storage (-20°C, avoiding freeze-thaw cycles) and validated protocols are critical for reproducibility. Overall, RBFOX1 antibodies remain indispensable for dissecting its contributions to cellular RNA processing and disease pathology.
×
