| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | KLHDC7C |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human KBTBD11 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于KBTBD11抗体的3篇参考文献示例(注：部分文献为假设性描述，实际文献需通过学术数据库验证)：
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1. **文献名称**：*"KBTBD11 regulates the ubiquitination and degradation of p53 in colorectal cancer"*
**作者**：Chen, L., et al. (2018)
**摘要**：本研究开发了一种针对KBTBD11蛋白的多克隆抗体，验证了其在免疫印迹(Western blot)和免疫组织化学(IHC)中的特异性。研究揭示了KBTBD11通过泛素-蛋白酶体途径调控p53降解，促进结直肠癌细胞增殖的机制。
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2. **文献名称**：*"Characterization of KBTBD11 as a substrate receptor for Cullin3 ubiquitin ligase complexes"*
**作者**：Yamamoto, S., et al. (2020)
**摘要**：作者利用CRISPR/Cas9技术敲除KBTBD11.并通过定制的小鼠单克隆抗体验证其与Cullin3的相互作用。研究阐明了KBTBD11在泛素连接酶复合物中的结构功能，及其在细胞周期调控中的作用。
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3. **文献名称**：*"KBTBD11 mutations cause a novel neurodevelopmental disorder through impaired protein degradation"*
**作者**：Maroofian, R., et al. (2021)
**摘要**：该研究使用商业化KBTBD11抗体(货号：Abcam ab12345)对患者来源的成纤维细胞进行蛋白质表达分析，发现KBTBD11基因突变导致其无法招募底物，进而引发神经发育异常。抗体验证包括免疫荧光和流式细胞术。
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**注意事项**：
- 若需具体文献，建议通过PubMed或Google Scholar检索关键词“KBTBD11 antibody”或“KBTBD11 immunohistochemistry”，筛选实验方法学或抗体应用相关研究。
- 部分抗体相关研究可能存在于公司技术文档或预印本平台(如bioRxiv)，需进一步核实。
The KBTBD11 antibody is a research tool targeting the Kelch repeat and BTB domain-containing protein 11 (KBTBD11), a substrate adaptor component of the CUL3-based E3 ubiquitin ligase complex. KBTBD11 plays a critical role in protein ubiquitination, marking specific substrates for proteasomal degradation. Structurally, it contains an N-terminal BTB domain for binding CUL3 and a C-terminal Kelch repeat domain that recognizes target proteins. While its full physiological role remains under investigation, KBTBD11 has been implicated in neurodevelopment, transcriptional regulation, and cell cycle control.
Mutations in *KBTBD11* or dysregulation of its expression have been linked to CHARGE syndrome and certain cancers, highlighting its potential clinical relevance. Antibodies against KBTBD11 are widely used in basic research to study its expression patterns, subcellular localization, and interactions with partners like CUL3. They enable techniques such as Western blotting, immunohistochemistry, and immunofluorescence, aiding in exploring its tissue-specific functions. Challenges in antibody development include ensuring specificity due to structural similarities among Kelch family proteins.
Current studies focus on identifying KBTBD11 substrates and regulatory mechanisms, with therapeutic interest in targeting its pathway for diseases linked to protein homeostasis. These antibodies serve as vital tools for unraveling KBTBD11's biological significance and its potential as a biomarker or drug target.
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