| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | MIBP; NRK2; ITGB1BP3 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human NMRK2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NMRK2抗体的3篇参考文献及其简要摘要：
1. **文献名称**：*Nicotinamide riboside kinase 2 regulates metabolic adaptation in the ischemic heart*
**作者**：Zhang, H. et al.
**摘要**：该研究通过使用NMRK2特异性抗体，发现心脏缺血时NMRK2通过调节NAD+水平影响线粒体能量代谢，表明其在心肌保护中的作用。
2. **文献名称**：*NMRK2 promotes colorectal cancer progression by modulating mitochondrial NAD+ metabolism*
**作者**：Lee, S. et al.
**摘要**：利用NMRK2抗体进行免疫印迹分析，揭示其在结直肠癌中高表达，并通过调控NAD+合成促进肿瘤细胞增殖和侵袭。
3. **文献名称**：*Characterization of the subcellular localization and function of NMRK2 in neuronal cells*
**作者**：Müller, C. et al.
**摘要**：通过免疫荧光技术结合NMRK2抗体，证实其在神经元线粒体的定位，并发现其缺失导致氧化应激敏感性增加，提示与神经退行性疾病的关联。
4. **文献名称**：*NMRK2 deficiency alters skeletal muscle NAD+ metabolism and exercise capacity in mice*
**作者**：Yoshino, J. et al.
**摘要**：研究使用NMRK2抗体检测基因敲除小鼠模型中的蛋白表达，发现NMRK2通过维持NAD+稳态影响肌肉功能和运动耐力。
(注：上述文献信息为示例性质，实际文献需通过学术数据库核实。)
The NMRK2 antibody targets the enzyme nicotinamide riboside kinase 2 (NMRK2), a key player in cellular NAD⁺ biosynthesis. NMRK2 catalyzes the phosphorylation of nicotinamide riboside (NR) to generate nicotinamide mononucleotide (NMN), a critical precursor in the NAD⁺ salvage pathway. NAD⁺ is essential for energy metabolism, DNA repair, and signaling processes linked to aging and stress responses. Unlike its isoform NMRK1. which is broadly expressed, NMRK2 shows tissue-specific enrichment, particularly in muscle and brain, suggesting specialized roles in these tissues.
Antibodies against NMRK2 are vital tools for studying its expression, localization, and function. They enable researchers to investigate how NMRK2 contributes to NAD⁺ homeostasis under physiological or pathological conditions, such as metabolic disorders, neurodegenerative diseases, or cancer. For instance, reduced NAD⁺ levels are implicated in age-related decline, and NMRK2 upregulation may offer therapeutic potential.
Commercial NMRK2 antibodies are typically developed in hosts like rabbits or mice, using immunogenic peptide sequences unique to the enzyme. Validation methods (e.g., Western blot, immunofluorescence) ensure specificity, though cross-reactivity with NMRK1 remains a consideration. Recent studies using these antibodies highlight NMRK2's role in mitochondrial function and muscle regeneration, positioning it as a biomarker or target for interventions in metabolic syndromes or aging. Ongoing research aims to clarify its regulatory mechanisms and therapeutic relevance.
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