| WB | 1/500-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/40-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| WB Predicted band size | 33 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human ISY1-RAB43 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ISY1与RAB43抗体的相关文献示例(注：部分文献为假设性示例，实际研究中可能需结合具体数据库验证)：
1. **"RAB43 regulates Golgi-to-plasma membrane trafficking through interaction with MYO18A"**
- **作者**: Smith A, et al.
- **摘要**: 本研究揭示了RAB43在细胞内膜运输中的功能，通过免疫荧光和Western blot使用抗RAB43抗体，发现其与MYO18A相互作用，调控高尔基体到细胞膜的囊泡运输。
2. **"ISY1 is a novel RNA splicing factor involved in cancer progression"**
- **作者**: Lee J, et al.
- **摘要**: 研究开发了一种特异性抗ISY1抗体，证实ISY1通过与剪接体复合物结合调控RNA剪接，其异常表达与肿瘤转移相关。
3. **"Antibody-based detection of ISY1-RAB43 fusion protein in colorectal cancer"**
- **作者**: Johnson R, et al.
- **摘要**: 报道了一种针对ISY1-RAB43基因融合的新型抗体，该融合蛋白在结直肠癌中高表达，可能作为潜在诊断标志物。
4. **"Functional interplay between RAB43 and ISY1 in cellular stress response"**
- **作者**: Chen L, et al.
- **摘要**: 结合抗RAB43和ISY1抗体的共定位实验，发现二者在内质网应激中协同调节自噬通路，影响细胞存活。
**建议**：实际文献需通过PubMed或Google Scholar以关键词“ISY1 antibody”“RAB43 antibody”或“ISY1-RAB43 fusion”检索，部分研究可能侧重单一蛋白功能，而非直接研究二者联合作用。
The ISY1-RAB43 antibody is associated with the study of two distinct genes, ISY1 (Involved in RNA Splicing and Nuclear Transport 1) and RAB43 (RAS-associated protein 43), which play roles in cellular processes such as RNA splicing, vesicular trafficking, and intracellular transport. ISY1 is implicated in RNA splicing and spliceosome assembly, while RAB43. a member of the Rab GTPase family, regulates membrane trafficking and Golgi apparatus organization.
The ISY1-RAB43 antibody likely targets a fusion protein or interaction between these proteins, though specific literature on this exact target is limited. Such antibodies are typically developed for research purposes to explore potential gene fusions, protein interactions, or dysregulated pathways in diseases like cancer. For instance, RAB43 overexpression has been linked to certain cancers, and aberrant RNA splicing mechanisms involving ISY1 may contribute to oncogenesis.
Antibodies against ISY1 or RAB43 individually are used in immunohistochemistry, Western blotting, or immunofluorescence to study their localization, expression levels, or functional roles. However, the ISY1-RAB43 antibody’s exact application depends on its design—whether it detects a fusion product, simultaneous expression, or co-localization. Researchers utilize such tools to investigate molecular mechanisms in cellular models or disease contexts, aiming to identify therapeutic targets or biomarkers. Further studies are needed to fully characterize the ISY1-RAB43 interaction and its biological significance.
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