| WB | 1/500-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | K5; DDD; EBS2; KRT5A; KRT5 |
| Entrez GeneID | 3852 |
| clone | 3E2F1 |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of CK5 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于IRX5抗体的3篇示例文献(注：以下内容为虚构示例，仅作格式参考)：
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1. **文献名称**：*IRX5 regulates cardiac ion channel expression through chromatin remodeling*
**作者**：Smith J, et al.
**摘要**：本研究利用IRX5特异性抗体进行ChIP-seq分析，揭示了IRX5在心脏发育中通过结合特定基因启动子区域调控离子通道(如KCNQ1)的表达，其缺失可能导致心律失常。
2. **文献名称**：*IRX5 promotes melanoma progression via Wnt/β-catenin signaling*
**作者**：Zhang L, et al.
**摘要**：通过IRX5抗体的免疫组化(IHC)和Western blot实验，作者发现IRX5在黑色素瘤中高表达，并通过激活Wnt通路促进肿瘤侵袭，提示其作为潜在治疗靶点。
3. **文献名称**：*Adipose-specific IRX5 knockout improves metabolic homeostasis*
**作者**：Kim S, et al.
**摘要**：研究使用IRX5抗体检测脂肪组织中IRX5蛋白水平，发现敲除IRX5可增强脂肪细胞分化并改善胰岛素敏感性，为代谢疾病机制提供新见解。
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如需真实文献，建议在PubMed或Google Scholar以“IRX5 antibody”或“IRX5 ChIP/IHC”为关键词检索，并筛选涉及分子机制或疾病应用的研究。
The IRX5 antibody is a tool used to detect the Iroquois homeobox protein 5 (IRX5), a transcription factor belonging to the Iroquois (Irx) gene family. This family plays critical roles in embryonic development, including tissue patterning, cell fate determination, and organogenesis. IRX5 is particularly implicated in heart development, neural differentiation, and limb formation. Studies highlight its involvement in regulating cardiac repolarization by modulating ion channel expression, linking it to arrhythmias. In cancer research, IRX5 has been associated with tumor progression, acting as either an oncogene or tumor suppressor depending on context—e.g., promoting breast cancer metastasis but suppressing colorectal cancer growth.
Commercially available IRX5 antibodies are typically produced in rabbits or mice using immunogenic peptides derived from conserved regions of the human IRX5 protein. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to study IRX5 expression, localization, and function in cells and tissues. Validation data often include reactivity across species (human, mouse, rat) and specificity confirmation via knockout controls. However, users should verify batch-specific performance, as variability in antibody quality can affect experimental reproducibility. Research applications span developmental biology, cardiovascular diseases, and oncology, with IRX5's dual roles in development and disease making it a compelling target for mechanistic and therapeutic studies.
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