| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | IP3KC; IP3-3KC |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human ITPKC |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于ITPKC抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*ITPKC functional polymorphism associated with Kawasaki disease susceptibility*
**作者**：Onouchi Y, et al.
**摘要**：该研究首次报道ITPKC基因多态性与川崎病易感性相关，通过抗体检测发现ITPKC蛋白表达水平影响T细胞活化，导致免疫反应异常。研究利用ITPKC抗体进行Western blot分析，验证基因突变对蛋白功能的调控作用。
2. **文献名称**：*Inositol 1.4.5-trisphosphate 3-kinase C modulates inflammatory T cell responses*
**作者**：Wen R, et al.
**摘要**：文章揭示ITPKC通过调控钙信号通路影响T细胞炎症反应，研究使用特异性ITPKC抗体进行免疫共沉淀实验，证实其与钙调蛋白的相互作用，为自身免疫疾病治疗提供潜在靶点。
3. **文献名称**：*Development and validation of a monoclonal antibody for human ITPKC detection*
**作者**：Li X, et al.
**摘要**：本研究开发并验证了一种高特异性抗人ITPKC单克隆抗体，通过ELISA、流式细胞术和免疫组化证明其可靠性，为ITPKC相关疾病的诊断和机制研究提供重要工具。
ITPKC (Inositol-trisphosphate 3-kinase C) is an enzyme encoded by the *ITPKC* gene, part of the inositol phosphate kinase family. It phosphorylates inositol 1.4.5-trisphosphate (IP3) to produce inositol 1.3.4.5-tetrakisphosphate (IP4), modulating intracellular calcium signaling—a critical pathway for immune cell activation, apoptosis, and gene expression. ITPKC acts as a negative regulator of T-cell receptor signaling by limiting excessive calcium influx, thereby preventing hyperimmune responses.
Research on ITPKC gained prominence due to its association with Kawasaki disease (KD), a childhood vasculitis. A 2008 study identified *ITPKC* polymorphisms as susceptibility factors for KD and coronary artery complications, linking reduced ITPKC activity to dysregulated T-cell activation and cytokine storms. This discovery highlighted its role in immune homeostasis and inflammatory diseases.
ITPKC antibodies are essential tools for studying its expression, localization, and function in immune cells and tissues. They enable detection via Western blot, immunohistochemistry, or flow cytometry, aiding research into calcium signaling disorders, autoimmune conditions, and cardiovascular pathologies. Commercial antibodies are typically validated for specificity against human or murine ITPKC isoforms. Ongoing studies explore ITPKC's interactions with calmodulin and other calcium-regulating proteins, broadening its relevance in cellular physiology and therapeutic targeting.
×
