| WB | 1/200-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | SWA; SWAP; SH3D1B; SH3P18; PRO2015 |
| WB Predicted band size | 193 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human ITSN2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ITSN2抗体的模拟参考文献示例(注：以下内容为虚构，仅作格式参考，实际文献请通过学术数据库查询)：
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1. **文献名称**：*ITSN2在乳腺癌中的异常表达及其临床意义*
**作者**：Smith A, et al.
**摘要**：本研究通过免疫组化(使用兔源ITSN2多克隆抗体)分析乳腺癌组织样本，发现ITSN2蛋白表达显著高于正常组织，且与肿瘤转移和预后不良相关，提示其可能作为潜在生物标志物。
2. **文献名称**：*ITSN2调控神经元突触形成的分子机制*
**作者**：Chen L, et al.
**摘要**：利用ITSN2特异性抗体进行Western blot和免疫荧光染色，发现敲低ITSN2会抑制海马神经元突触小泡的内吞过程，表明其在突触可塑性中起关键作用。
3. **文献名称**：*ITSN2与EGFR信号通路的相互作用研究*
**作者**：Johnson R, et al.
**摘要**：通过免疫共沉淀(ITSN2小鼠单克隆抗体)和质谱分析，证实ITSN2直接结合EGFR并调控其内吞降解，影响下游MAPK信号通路的激活。
4. **文献名称**：*ITSN2在阿尔茨海默病模型中的表达变化*
**作者**：Lee H, et al.
**摘要**：采用ITSN2抗体检测转基因小鼠脑组织，发现其蛋白水平随β-淀粉样蛋白沉积增加而下调，提示ITSN2可能参与神经退行性病变的病理过程。
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如需真实文献，建议通过PubMed、Google Scholar等平台检索关键词“ITSN2 antibody”或“Intersectin 2 antibody”，并筛选涉及实验应用的论文。
Intersectin-2 (ITSN2) is a multidomain scaffolding protein belonging to the intersectin family, primarily involved in regulating endocytosis, intracellular trafficking, and signal transduction. It contains conserved Eps15 homology (EH) domains and Src homology 3 (SH3) domains, enabling interactions with various partners to coordinate membrane dynamics, cytoskeletal reorganization, and receptor signaling. ITSN2 is expressed in diverse tissues, with notable roles in neuronal development, synaptic vesicle recycling, and cellular proliferation. Dysregulation of ITSN2 has been implicated in neurological disorders, cancer progression, and cardiovascular diseases, highlighting its functional versatility.
ITSN2 antibodies are essential tools for detecting and analyzing the expression, localization, and molecular interactions of ITSN2 in both physiological and pathological contexts. These antibodies are widely used in techniques such as Western blotting, immunofluorescence, immunohistochemistry, and co-immunoprecipitation to study ITSN2's involvement in cellular processes like clathrin-mediated endocytosis, Ras-MAPK signaling, and PI3K-Akt pathway modulation. Polyclonal antibodies offer broad epitope recognition, while monoclonal antibodies provide high specificity. Validation via knockout controls or siRNA knockdown is critical to ensure antibody reliability, particularly given ITSN2's structural similarity to ITSN1. its paralog. Research utilizing ITSN2 antibodies continues to uncover its dual roles as a tumor suppressor or promoter, depending on cellular context, making it a focal point in therapeutic target exploration.
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