| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPS19 |
| Uniprot No | P39019 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-145aa |
| 氨基酸序列 | PGVTVKDVN QQEFVRALAA FLKKSGKLKV PEWVDTVKLA KHKELAPYDE NWFYTRAAST ARHLYLRGGA GVGSMTKIYG GRQRNGVMPS HFSRGSKSVA RRVLQALEGL KMVEKDQDGG RKLTPQGQRD LDRIAGQVAA ANKKH |
| 预测分子量 | 16 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于RPS19重组蛋白的经典文献摘要概括(基于真实研究领域内容，但具体标题和作者为领域内代表性研究示例)：
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1. **文献名称**: "RPS19 mutations in Diamond-Blackfan anemia impair ribosomal protein function by reducing protein stability"
**作者**: Gazda HT, et al.
**摘要**: 本研究通过重组表达野生型和突变型RPS19蛋白，发现DBA相关突变导致蛋白稳定性降低，影响核糖体组装。实验显示突变体在体外降解加速，与患者细胞中40S核糖体亚基缺失相关。
2. **文献名称**: "Structural insights into the role of RPS19 in 40S ribosomal subunit maturation"
**作者**: Choesmel V, et al.
**摘要**: 通过重组人源RPS19蛋白的晶体结构分析，揭示了其与18S rRNA的结合位点，并证明其作为核糖体组装因子的关键作用。研究为理解RPS19突变导致核糖体生物合成障碍提供了结构基础。
3. **文献名称**: "Recombinant RPS19 protein rescues erythroid differentiation in cellular models of Diamond-Blackfan anemia"
**作者**: Flygare J, et al.
**摘要**: 利用重组RPS19蛋白处理DBA患者来源的造血干细胞，发现其部分恢复红细胞分化能力，表明外源性RPS19可能通过补偿机制缓解核糖体功能缺陷，为潜在治疗策略提供依据。
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*注：以上文献标题和作者为领域内代表性研究方向示例，具体内容需通过PubMed或Google Scholar检索真实文献确认。*
**Background of RPS19 Recombinant Protein**
RPS19 (Ribosomal Protein S19) is a critical component of the 40S ribosomal subunit, playing essential roles in ribosome biogenesis and protein synthesis. As part of the ribosomal machinery, RPS19 contributes to the assembly and stability of ribosomal subunits, ensuring accurate translation of mRNA into functional proteins. Beyond its canonical role in translation, RPS19 has been implicated in diverse cellular processes, including cell proliferation, differentiation, and apoptosis, highlighting its pleiotropic functions.
Mutations in the *RPS19* gene are strongly associated with Diamond-Blackfan anemia (DBA), a rare inherited bone marrow failure syndrome characterized by defective erythropoiesis and developmental abnormalities. Approximately 25% of DBA cases are linked to *RPS19* mutations, which disrupt ribosome assembly, leading to ribosomal stress and p53-mediated cell cycle arrest in erythroid progenitors. This connection has positioned RPS19 as a key molecule for studying ribosomopathies and hematopoiesis.
Recombinant RPS19 protein is produced using biotechnological systems, such as *E. coli* or mammalian expression systems, to ensure high purity and biological activity. Its production typically involves cloning the *RPS19* gene into expression vectors, followed by purification via affinity chromatography. The recombinant protein serves as a vital tool for functional studies, enabling researchers to investigate its interactions with ribosomal components, its role in ribosome assembly, and its extraribosomal functions in disease models.
In therapeutic research, recombinant RPS19 holds potential for gene therapy in DBA and as a target for small-molecule drugs aimed at restoring ribosomal function. Additionally, it is used in diagnostic assays to detect *RPS19* mutations and evaluate their impact on protein stability. Ongoing studies continue to explore its involvement in cancer, where ribosomal dysfunction may influence tumor progression, further expanding its biomedical relevance.
Overall, RPS19 recombinant protein bridges basic ribosome biology and clinical applications, offering insights into both congenital disorders and broader cellular mechanisms.
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