| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/40-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | C3G; GRF2 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human RAPGEF1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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1. **"RAPGEF1 mediates PDGF-BB-induced cell surface translocation of PDGFRβ"**
- **作者**: Wu et al.
- **摘要**: 研究利用RAPGEF1特异性抗体揭示了其在血小板衍生生长因子(PDGF)信号中调控PDGFRβ膜转运的机制，证实RAPGEF1通过激活Rap1促进细胞迁移。
2. **"Role of RAPGEF1 in cAMP-dependent exocytosis in pancreatic β-cells"**
- **作者**: Sugita et al.
- **摘要**: 通过RAPGEF1抗体阻断实验，证明RAPGEF1介导cAMP信号通路，调控胰岛素分泌颗粒的胞吐作用，为糖尿病治疗提供潜在靶点。
3. **"RAPGEF1 is required for endothelial barrier function through Rap1 activation"**
- **作者**: Gloerich et al.
- **摘要**: 使用RAPGEF1抗体进行免疫沉淀及基因敲除实验，发现其通过激活Rap1增强血管内皮细胞连接稳定性，维持屏障功能。
4. **"Antibody-based profiling of RAPGEF1 expression in human cancers"**
- **作者**: Lee et al.
- **摘要**: 研究开发高特异性RAPGEF1抗体，分析其在多种肿瘤中的表达差异，提示RAPGEF1可能作为癌症预后标志物。
注：以上为模拟参考文献，实际文献需通过PubMed/Google Scholar检索确认。建议使用关键词“RAPGEF1 antibody”、“RAPGEF1 function”查找最新研究。
The RAPGEF1 antibody is a key tool for studying the Ras-associated protein Rap guanine nucleotide exchange factor 1 (RAPGEF1), also known as C3G (CRK complex component). RAPGEF1 is a member of the RapGEF family and functions as a guanine nucleotide exchange factor (GEF) that activates Rap GTPases by promoting the exchange of GDP for GTP. This protein plays critical roles in intracellular signaling pathways regulating cell adhesion, migration, proliferation, and differentiation. It interacts with adaptor proteins like CRK and participates in signaling cascades triggered by growth factors, cytokines, and integrin-mediated cell-matrix interactions.
RAPGEF1 is implicated in diverse physiological processes, including neuronal development, cardiovascular function, and immune responses, as well as pathological conditions such as cancer metastasis and inflammation. Antibodies targeting RAPGEF1 are widely used in research to detect its expression, localization, and post-translational modifications via techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and co-immunoprecipitation (Co-IP). These antibodies help elucidate its regulatory mechanisms in diseases and its crosstalk with pathways like MAPK/ERK and PI3K/AKT.
Commercial RAPGEF1 antibodies are typically validated for specificity and reactivity across human, mouse, or rat samples. Researchers rely on these reagents to explore RAPGEF1's role in cellular signaling dynamics and its potential as a therapeutic target in oncology and other diseases.
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