| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | FAST1; FAST-1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human FOXH1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于FOXH1抗体的3篇参考文献，简要总结如下：
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1. **文献名称**: "Foxh1 is essential for development of the anterior heart field"
**作者**: von Both, I. et al.
**摘要**: 该研究利用Foxh1特异性抗体进行胚胎组织免疫染色，发现Foxh1在前心区发育中不可或缺，并通过Western blot验证其在心脏祖细胞中的表达，证明其通过调控Nodal信号通路影响心脏形成。
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2. **文献名称**: "The role of FoxH1 in TGF-β-dependent transcriptional responses"
**作者**: Watanabe, Y. & Whitman, M.
**摘要**: 研究通过FoxH1抗体进行染色质免疫共沉淀(ChIP)，揭示FoxH1作为TGF-β信号下游的转录共激活因子，直接结合靶基因启动子(如Mix2)，并依赖Smad2/4复合体发挥作用。
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3. **文献名称**: "Foxh1 mediates nodal signaling during early vertebrate embryogenesis"
**作者**: Chen, C. & Shen, M.M.
**摘要**: 利用FoxH1抗体进行斑马鱼胚胎免疫荧光定位，证实FoxH1在早期胚胎中响应Nodal信号，调控中胚层和内胚层分化，抗体特异性通过siRNA敲降实验验证。
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**备注**：上述文献为示例，实际引用时需核实具体来源及细节。若需精准文献，建议通过PubMed或Web of Science以“FOXH1 antibody”为关键词检索近年研究。
The FOXH1 antibody is a key tool for studying the Forkhead box H1 (FOXH1) protein, a member of the Forkhead box (FOX) family of transcription factors characterized by a conserved winged-helix DNA-binding domain. FOXH1 plays a critical role in early embryonic development, particularly in mediating signaling pathways such as TGF-β and Nodal. These pathways are essential for establishing the body plan, left-right asymmetry, and germ layer specification (e.g., mesoderm formation). FOXH1 acts as a transcriptional coactivator, binding to SMAD2/3-SMAD4 complexes to regulate target genes like *MIXL1* and *LEFTY2*.
FOXH1 antibodies are widely used in research to detect and quantify FOXH1 expression in models ranging from zebrafish and Xenopus to mammalian cells. They enable techniques like Western blotting, immunohistochemistry, and chromatin immunoprecipitation (ChIP) to explore FOXH1's localization, interactions, and regulatory mechanisms. Commercial FOXH1 antibodies are typically raised in rabbits or mice, with specificity validated via knockout controls or siRNA knockdown.
Dysregulation of FOXH1 has been linked to developmental disorders and cancers, including gastric and colorectal tumors, where it may promote metastasis. Researchers also utilize these antibodies in stem cell studies to assess pluripotency and differentiation. However, variability in antibody performance (e.g., cross-reactivity, batch differences) necessitates careful optimization of experimental conditions. Major suppliers include Abcam, Cell Signaling Technology, and Santa Cruz Biotechnology, each offering antibodies tailored for specific applications.
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