首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠胰高血糖素样肽2(Glp-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Glp2含量。Glp2即胰高血糖素样肽-2，由肠道L细胞分泌。其作用机制主要是与特异性受体结合，可促进肠黏膜生长、改善肠道屏障功能、调节消化液分泌等。目前被广泛研究用于治疗短肠综合征、肠道损伤等肠道疾病。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于营养学、消化系统疾病模型研究及药物干预实验中的Glp - 2动态监测。为研究肠道屏障功能、代谢综合征及肠上皮细胞增殖机制提供可靠的实验工具，满足体外科研实验中低丰度生物标志物的定量需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：Glp2
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat GLP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 89 | |||||
| Range % | 82-95 | ||||||
| 1:2 | Average % | 98 | |||||
| Range % | 93-103 | ||||||
| 1:4 | Average % | 97 | |||||
| Range % | 90-104 | ||||||
| 1:8 | Average % | 86 | |||||
| Range % | 82-91 | ||||||
回收率：
| The recovery of rat GLP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 92 | 87-99 | |||||
| EDTA plasma (n=4) | 99 | 96-104 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected10002.0572.0522.0551.9125001.6031.6331.6181.4752501.2121.2281.2201.0771250.8460.9110.8790.73662.50.5590.5430.5510.40831.20.3320.3510.3420.19915.60.2520.2650.2590.11600.1420.1440.143 | |||||||
货期：3-5 working days
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