首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠抑胃肽(GIP)酶联免疫试剂盒双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Gip含量。GIP（葡萄糖依赖性促胰岛素多肽）是一种由肠道K细胞分泌的肠促胰岛素，主要分布在十二指肠和近端空肠，对食物摄取后胰岛素的分泌起到重要调节作用。GIP通过与其受体结合，激活信号通路，刺激胰岛β细胞分泌胰岛素，同时在胰岛α细胞中促进胰高血糖素的分泌，从而在血糖调节中发挥智能调节作用。GIP的研究对于理解糖尿病和肥胖等代谢疾病的发病机制具有重要意义。试剂盒检测范围为6.25 pg/mL-400 pg/mL，可满足科研场景中对GIP动态变化的检测需求，例如研究肠道激素分泌调控机制、评估营养干预或药物处理对大鼠代谢状态的影响等。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：GipGastric inhibitory polypeptide ELISA kit; GIP ELISA kit; Glucose-dependent insulinotropic polypeptide ELISA kit
缩写：Gip
Uniprot No.：Q06145
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat Gip in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:1 | Average % | 93 | |
| Range % | 87-96 | ||
| 1:2 | Average % | 99 | |
| Range % | 93-105 | ||
| 1:4 | Average % | 90 | |
| Range % | 85-94 | ||
| 1:8 | Average % | 84 | |
| Range % | 80-88 |
回收率：
| The recovery of rat Gip spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 92-102 | ||||
| EDTA plasma (n=4) | 95 | 90-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 4002.8122.7322.7722.599 2001.9651.9011.9331.760 1001.2151.2471.2311.058 500.7130.7530.7330.560 250.5160.5310.5240.351 12.50.4330.4090.4210.248 6.250.2570.2410.2490.076 00.1760.1690.173 | ||||||
货期：3-5 working days
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