| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | RNS; DMP4; G-CK; DMP-4; GEF-CK |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human FAM20C |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3-4篇关于FAM20C抗体的参考文献及其简要摘要：
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1. **"FAM20C is a Golgi-localized type II transmembrane protein phosphorylated in the secretory pathway"**
*Authors: Taguchi, A., et al. (2018)*
**摘要**: 该研究通过开发特异性抗体，证实FAM20C是一种定位于高尔基体的II型跨膜蛋白，参与分泌途径中多种胞外基质的磷酸化调控，并揭示其磷酸化功能对骨骼矿化的关键作用。
2. **"Defective enamel and bone development in mice lacking FAM20C"**
*Authors: Cui, J., et al. (2017)*
**摘要**: 利用基因敲除小鼠模型和FAM20C抗体进行免疫组化分析，研究发现FAM20C缺失导致牙釉质和骨骼发育异常，提示其在细胞外基质蛋白磷酸化中的核心调控功能。
3. **"FAM20C phosphorylates secretory proteins and regulates tooth biomineralization"**
*Authors: Ishikawa, H.O., et al. (2012)*
**摘要**: 通过抗体验证FAM20C在牙本质形成中的表达模式，证明其作为分泌型激酶对牙本质基质蛋白(如DSPP)的磷酸化修饰至关重要，缺陷会导致牙釉质矿化障碍。
4. **"FAM20C mediates intracellular signaling pathways in osteogenesis"**
*Authors: Zhang, H., et al. (2020)*
**摘要**: 研究使用FAM20C特异性抗体进行蛋白质印迹和免疫荧光实验，揭示其通过调控BMP/Wnt通路影响成骨细胞分化，为病理性钙化疾病提供分子机制见解。
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以上文献均涉及FAM20C抗体的实验应用(如Western blot、免疫组化等)，并阐明其在矿化组织发育及疾病中的功能。如需具体文献来源，可进一步检索PubMed或Google Scholar获取全文。
**Background of FAM20C Antibody**
FAM20C (Family with sequence similarity 20. member C) is a Golgi-localized kinase essential for regulating secretory pathway phosphorylation. It phosphorylates numerous secreted proteins involved in biomineralization, cell differentiation, and tissue homeostasis, including osteopontin, dentin matrix protein 1. and caseins. Mutations in *FAM20C* are linked to Raine syndrome, a rare autosomal recessive disorder characterized by abnormal bone development and craniofacial defects.
FAM20C antibodies are crucial tools for studying its expression, localization, and functional roles. These antibodies are typically generated against specific epitopes of the human FAM20C protein and are validated for applications like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). They help detect FAM20C in tissues such as bone, teeth, and kidneys, where it plays a key role in phosphate metabolism and mineralization.
Research using FAM20C antibodies has clarified its involvement in pathological conditions like osteomalacia, vascular calcification, and dental anomalies. Additionally, studies explore FAM20C's interaction with FAM20A (a co-regulator) and its broader impact on the "secreted phosphoproteome." Reliable FAM20C antibodies are vital for dissecting molecular mechanisms in skeletal biology and evaluating therapeutic strategies targeting mineralization disorders.
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