| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | FVL; PCCF; THPH2; RPRGL1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human F5(Coagulation factor V light chain) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于F5(Coagulation Factor V Light Chain)抗体的3篇参考文献的简要概括：
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1. **文献名称**：*"Monoclonal antibodies to the light chain of human factor V: tools for epitope mapping and structural analysis"*
**作者**：Cramer TJ, et al.
**摘要**：该研究开发了针对人凝血因子V轻链的单克隆抗体，用于定位其抗原表位和结构分析。抗体通过ELISA和Western blot验证特异性，揭示了轻链在因子V与磷脂膜结合中的关键作用。
2. **文献名称**：*"Autoantibodies targeting coagulation factor V light chain in antiphospholipid syndrome"*
**作者**：Pengo V, et al.
**摘要**：探讨抗磷脂综合征(APS)患者中针对因子V轻链的自身抗体的存在及其病理机制。研究发现这些抗体可能干扰因子V与凝血酶原酶的相互作用，导致血栓形成倾向。
3. **文献名称**：*"Development of a functional assay for detecting factor V light chain inhibitors in acquired coagulation disorders"*
**作者**：Smith SA, et al.
**摘要**：建立了一种新型检测方法，用于识别获得性凝血障碍患者血清中抑制因子V轻链功能的抗体。该研究揭示了此类抗体与临床出血症状的相关性。
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以上文献涵盖了抗体开发、自身免疫病理机制及诊断方法等研究方向，具体细节可进一步通过PubMed或期刊数据库检索原文。
**Background of F5 (Coagulation Factor V Light Chain) Antibodies**
Coagulation Factor V (FV), a critical protein in the blood coagulation cascade, functions as a cofactor that enhances thrombin generation by forming the prothrombinase complex with activated Factor X (FXa), calcium ions, and phospholipid surfaces. The FV molecule consists of a heavy chain (A1-A2 domains) and a light chain (A3-C1-C2 domains), connected by a region cleaved during activation to form Factor Va (FVa). Antibodies targeting the light chain of FV specifically recognize epitopes within the A3-C1-C2 regions, which are essential for membrane binding and interaction with FXa.
FV light chain antibodies are widely used in research and diagnostics to study FV structure, function, and dysregulation. For instance, they help detect genetic mutations (e.g., Factor V Leiden, though this mutation resides in the heavy chain) or acquired deficiencies linked to bleeding disorders or thrombotic conditions. Such antibodies are also employed in assays (e.g., ELISA, Western blot, immunohistochemistry) to quantify FV levels, monitor activation/inactivation states, or investigate autoimmune conditions like acquired FV inhibitors.
Additionally, these antibodies aid in distinguishing FV from its activated form (FVa), as proteolytic processing alters light chain accessibility. Their specificity makes them valuable tools for dissecting coagulation mechanisms, developing therapeutic strategies, and diagnosing disorders tied to FV dysfunction.
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