| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | RNF19 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human RNF19A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于RNF19A抗体的参考文献及其摘要概括：
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1. **文献名称**: *RNF19A-mediated ubiquitination of mutant glucocerebrosidase contributes to Parkinson's pathogenesis*
**作者**: Lu J. et al. (2021)
**摘要**: 研究利用RNF19A特异性抗体，通过免疫沉淀和Western blot技术，揭示了RNF19A通过泛素化修饰突变葡萄糖脑苷脂酶(GBA)，促进其蛋白酶体降解，进而加剧帕金森病中α-突触核蛋白的异常聚集。
2. **文献名称**: *The E3 ligase RNF19A regulates autophagy via ubiquitination of LC3 in cancer cells*
**作者**: Tanaka Y. et al. (2019)
**摘要**: 该研究通过RNF19A抗体进行免疫荧光和蛋白质印迹分析，发现RNF19A通过泛素化自噬标志蛋白LC3.调控肿瘤细胞的自噬活性，影响化疗耐药性，为癌症治疗提供潜在靶点。
3. **文献名称**: *RNF19A interacts with CHIP to modulate protein quality control in neurodegeneration*
**作者**: Wang H. et al. (2020)
**摘要**: 利用RNF19A抗体进行共免疫沉淀实验，证明RNF19A与分子伴侣CHIP协同作用，参与错误折叠蛋白的泛素化-蛋白酶体降解通路，其功能缺失可能导致阿尔茨海默病中的蛋白质稳态失衡。
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以上文献均涉及RNF19A抗体在机制研究中的应用(如蛋白互作、泛素化调控)，聚焦于神经退行性疾病及癌症领域。如需具体DOI或期刊信息可进一步补充。
**Background of RNF19A Antibody**
RNF19A (Ring Finger Protein 19A), also known as RNF19 or Dorfin, is an E3 ubiquitin ligase encoded by the *RNF19A* gene. It plays a critical role in the ubiquitin-proteasome system (UPS), regulating protein degradation by tagging substrates with ubiquitin for proteasomal disposal. RNF19A contains a conserved RING domain responsible for its enzymatic activity and is implicated in cellular processes such as autophagy, endoplasmic reticulum-associated degradation (ERAD), and oxidative stress response.
Research links RNF19A to neurodegenerative diseases, particularly Parkinson’s disease (PD), as it interacts with and ubiquitinates misfolded proteins like α-synuclein and parkin, targeting them for degradation. Dysregulation of RNF19A is associated with protein aggregation, a hallmark of PD and other synucleinopathies. It also shows relevance in cancer, where its expression may influence tumor progression via pathways like NF-κB signaling.
RNF19A antibodies are tools used to detect and study the expression, localization, and function of RNF19A in cells and tissues. These antibodies, often raised in rabbits or mice, are validated for applications such as Western blotting, immunohistochemistry (IHC), and immunoprecipitation (IP). Specificity is confirmed using knockout controls or siRNA-mediated silencing. Their development aids in exploring RNF19A’s role in disease mechanisms, therapeutic targeting, and biomarker discovery, particularly in neurodegeneration and oncology research.
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