| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | ACG; ZIZ2; bB128O4.1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human DOCK11 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于DOCK11抗体的3篇参考文献示例(注：部分文献信息为假设性概括，具体文献需根据实际数据库查询)：
1. **文献名称**: "DOCK11 regulates T cell migration through Cdc42 activation and filopodia formation"
**作者**: Yamamoto et al.
**摘要**: 研究利用特异性DOCK11抗体，通过免疫沉淀和Western blot技术，揭示了DOCK11在T细胞中激活Cdc42 GTP酶并促进细胞迁移的机制，抗体验证了其在免疫突触中的定位。
2. **文献名称**: "Characterization of a novel monoclonal antibody against DOCK11 for cancer metastasis studies"
**作者**: Chen et al.
**摘要**: 报道了一种高特异性抗DOCK11单克隆抗体的开发，通过免疫组化及流式细胞术验证其在多种癌细胞系中的表达，并发现DOCK11高表达与肿瘤侵袭性相关。
3. **文献名称**: "DOCK11 interacts with RHO GTPases in endothelial cell angiogenesis"
**作者**: Gupta & Lee
**摘要**: 研究通过DOCK11抗体进行免疫共沉淀和共聚焦显微分析，证明DOCK11与RhoA/Rac1的相互作用在血管生成中的作用，抗体特异性在基因敲除模型中验证。
4. **文献名称**: "DOCK11 deficiency disrupts spermatogenesis: Insights from antibody-based profiling in mouse testes"
**作者**: Rodriguez et al.
**摘要**: 使用DOCK11抗体进行组织免疫荧光和蛋白质印迹分析，发现DOCK11在精子形成过程中调控细胞极性，基因缺失导致生殖细胞发育异常。
(注：以上文献为示例，实际文献需通过PubMed或Google Scholar检索确认。)
The DOCK11 antibody is a tool used to detect and study Dedicator of Cytokinesis 11 (DOCK11), a protein belonging to the DOCK family of guanine nucleotide exchange factors (GEFs). DOCK11. also known as Zizimin2. specifically activates the Rho GTPase CDC42 by catalyzing the exchange of GDP for GTP, regulating cellular processes like cytoskeletal reorganization, cell migration, and polarity. It plays critical roles in immune cell trafficking, neuronal development, and cancer metastasis.
DOCK11 antibodies are essential for investigating its expression, localization, and function in various biological contexts. They are widely used in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to explore DOCK11's involvement in signaling pathways and disease mechanisms. For instance, studies have linked DOCK11 dysregulation to pathologies like immunodeficiency disorders, autoimmune diseases, and tumor progression.
Commercial DOCK11 antibodies are typically raised in hosts like rabbits or mice, targeting specific epitopes within the protein's conserved DOCK homology region (DHR-2 domain) or other functional regions. Researchers must validate these antibodies for specificity and cross-reactivity, as DOCK family members share structural similarities. Recent advances in structural biology and genetic models (e.g., CRISPR/Cas9) have further clarified DOCK11's roles, driving demand for reliable antibodies in both basic and translational research.
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