| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | SGY; CT34; SGY1; SGY-1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human DKKL1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是与DKKL1抗体相关的参考文献示例(内容为模拟，建议根据实际文献调整)：
1. **文献名称**: *DKKL1 is essential for sperm motility and male fertility in mice*
**作者**: Li et al.
**摘要**: 研究通过基因敲除和DKKL1特异性抗体验证，发现DKKL1在小鼠精子成熟中起关键作用，其缺失导致精子运动缺陷和不育。
2. **文献名称**: *Characterization of a novel monoclonal antibody against human DKKL1 for cancer biomarker studies*
**作者**: Smith JR, et al.
**摘要**: 开发并验证了一种高特异性抗人DKKL1单克隆抗体，应用于ELISA和免疫组化，显示DKKL1在多种肿瘤组织中的差异表达。
3. **文献名称**: *DKKL1 regulates neurogenesis through Wnt/β-catenin signaling modulation*
**作者**: Chen X, et al.
**摘要**: 利用DKKL1多克隆抗体进行Western blot和免疫荧光，证明其在神经干细胞分化中通过调控Wnt通路影响神经元生成。
4. **文献名称**: *DKKL1 as a potential therapeutic target in autoimmune disorders*
**作者**: Gonzalez S, et al.
**摘要**: 研究使用商业化DKKL1抗体进行流式细胞术，发现DKKL1在调节性T细胞中高表达，可能参与自身免疫疾病的免疫抑制机制。
**注意**：以上为模拟示例，实际文献需通过PubMed、Google Scholar等平台以关键词“DKKL1 antibody”或“DKKL1 AND (immunohistochemistry/Western/flow cytometry)”检索。若研究较少，可扩展至相关功能研究并注明抗体应用部分。
DKKL1 (Dickkopf-like protein 1), also known as Soggy, is a secreted glycoprotein belonging to the Dickkopf (DKK) family. While it shares structural homology with other DKK members, particularly DKK1. its functional role appears distinct. Unlike canonical DKK proteins that primarily regulate Wnt signaling by binding to LRP5/6 co-receptors, DKKL1 lacks the conserved cysteine-rich domain critical for this interaction, suggesting divergent mechanisms. It is highly expressed in the testis, particularly in germ cells, and has been implicated in spermatogenesis, embryogenesis, and immune modulation. Studies also link DKKL1 to neurodevelopment, where it may influence neuronal differentiation and synaptic plasticity.
DKKL1 antibodies are essential tools for investigating its expression patterns, interactions, and biological functions. These antibodies enable detection via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Research applications include exploring DKKL1's role in male infertility, neurodevelopmental disorders, and cancer, where aberrant expression has been observed in gliomas and testicular tumors. Some antibodies target specific epitopes, such as the N-terminal region, and are validated for specificity using knockout controls. As DKKL1 remains less characterized than other DKK proteins, high-quality antibodies are crucial for advancing mechanistic insights into its unique physiological and pathological contributions.
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