| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | 44M2.4; SCCRO3 |
| WB Predicted band size | 34 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human DCUN1D3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于DCUN1D3抗体的模拟参考文献(部分信息可能需进一步验证)：
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1. **文献名称**：*DCUN1D3 interacts with Cullin-RING ubiquitin ligase complexes to regulate substrate ubiquitination in squamous cell carcinoma*
**作者**：Yokoi S, et al.
**摘要**：本研究揭示了DCUN1D3作为Cullin-RING泛素连接酶复合体(CRL)的调控因子，通过免疫共沉淀(Co-IP)和Western blot实验验证其与CUL1的相互作用。研究发现，DCUN1D3抗体在食道鳞癌组织中的高表达与患者预后不良相关，并促进肿瘤细胞增殖和转移。
2. **文献名称**：*The role of DCUN1D3 in DNA damage response and chemoresistance*
**作者**：Li X, Wang H, Zhang R.
**摘要**：通过免疫组化(IHC)和siRNA敲降实验，本文证明DCUN1D3通过调控BRCA1泛素化影响DNA损伤修复通路。使用特异性DCUN1D3抗体检测发现，其在化疗耐药型卵巢癌中表达上调，提示其作为潜在治疗靶点。
3. **文献名称**：*DCUN1D3 deficiency induces apoptosis via destabilizing mitochondrial membrane proteins*
**作者**：Sakamoto K, Watson IR.
**摘要**：研究利用CRISPR/Cas9敲除DCUN1D3基因，结合流式细胞术和Western blot(使用兔源DCUN1D3抗体)发现，DCUN1D3缺失导致线粒体膜蛋白降解，激活caspase-3依赖的凋亡通路，抑制肺癌细胞生长。
4. **文献名称**：*Antibody-based profiling of DCUN1D3 in tumor microenvironment and immunotherapy response*
**作者**：Zhang Y, et al.
**摘要**：通过多色荧光免疫组化(使用商业化DCUN1D3单克隆抗体)，分析DCUN1D3在肿瘤微环境中的表达模式。结果显示，DCUN1D3高表达与CD8+ T细胞浸润减少相关，提示其可能通过免疫抑制机制影响PD-1抑制剂疗效。
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**注意事项**：以上文献信息为模拟生成，实际引用请通过PubMed、Google Scholar等平台以**"DCUN1D3 antibody"**或**"DCUN1D3 function"**为关键词检索核实。建议关注近年(2018-2023)的肿瘤学或细胞生物学期刊，如*Nature Cell Biology*、*Cancer Research*等。
The DCUN1D3 (Defective in Cullin Neddylation 1 Domain Containing 3) antibody is a tool used to detect and study the DCUN1D3 protein, a member of the DCNL family involved in the neddylation pathway. Neddylation, a post-translational modification similar to ubiquitination, regulates the activity of Cullin-RING E3 ubiquitin ligases (CRLs), which are critical for protein degradation and cellular processes like cell cycle progression, DNA repair, and signaling. DCUN1D3 specifically facilitates the transfer of NEDD8 (a ubiquitin-like protein) to Cullin proteins, enhancing CRL assembly and substrate recognition.
Abnormal DCUN1D3 expression has been linked to various cancers, including lung and colorectal cancer, where it may act as an oncogene by promoting cell proliferation or metastasis. Its role in modulating CRL activity also implicates it in tumorigenesis and therapeutic resistance. The DCUN1D3 antibody is widely used in research to assess protein expression levels, localization (via techniques like Western blotting, immunohistochemistry, or immunofluorescence), and interactions in cellular pathways. Both monoclonal and polyclonal variants are available, with specificity validated for human, mouse, or other model organisms. Understanding DCUN1D3’s function through antibody-based studies could reveal novel biomarkers or targets for cancer therapy, emphasizing its importance in molecular and translational research.
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