| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human CYP2R1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于CYP2R1抗体的3篇参考文献示例(基于现有研究主题的合理推测，具体文献可能需要进一步验证)：
---
1. **文献名称**：*"Cloning and characterization of a novel human cytochrome P450 gene (CYP2R1) encoding vitamin D 25-hydroxylase"*
**作者**：Shinkyo R, et al.
**摘要**：该研究首次克隆并鉴定了人类CYP2R1基因，证实其编码维生素D 25-羟化酶。研究通过体外表达和抗体检测，证明CYP2R1蛋白在肝脏中特异性表达，并具有催化维生素D转化为25-羟基维生素D的活性。
---
2. **文献名称**：*"Genetic evidence that the human CYP2R1 enzyme is a key vitamin D 25-hydroxylase"*
**作者**：Cheng JB, et al.
**摘要**：通过基因分析和体外酶活性实验，研究验证了CYP2R1在维生素D代谢中的核心作用。研究使用特异性抗体进行Western blot和免疫组化，揭示了CYP2R1在肝细胞中的定位及表达水平与维生素D代谢能力的相关性。
---
3. **文献名称**：*"Vitamin D deficiency and CYP2R1 polymorphisms: implications for clinical diagnostics"*
**作者**：Wang TJ, et al.
**摘要**：该临床研究探讨了CYP2R1基因多态性与维生素D缺乏的关系，并利用CYP2R1抗体检测患者肝脏样本中的蛋白表达水平。结果表明，CYP2R1表达降低与特定基因突变及维生素D代谢障碍显著相关。
---
**注**：以上文献信息为示例性模拟，实际引用需根据具体研究通过学术数据库(如PubMed、Web of Science)检索确认。若需精准文献，建议补充关键词(如“antibody validation”“immunohistochemistry”等)进一步筛选。
The CYP2R1 antibody is a crucial tool for studying the CYP2R1 enzyme, a member of the cytochrome P450 superfamily primarily expressed in the liver. CYP2R1 plays a pivotal role in vitamin D metabolism by catalyzing the hydroxylation of vitamin D3 (cholecalciferol) to 25-hydroxyvitamin D3 [25(OH)D3], the major circulating form of vitamin D and a key biomarker of vitamin D status. This enzyme’s activity is essential for maintaining calcium homeostasis, bone health, and immune function. Mutations in the CYP2R1 gene are linked to hereditary vitamin D deficiency disorders, underscoring its physiological significance.
CYP2R1 antibodies are widely used in research to detect and quantify CYP2R1 protein expression in tissues, particularly in hepatic and extrahepatic studies. They enable investigations into CYP2R1 regulation under various pathological conditions, such as chronic liver disease, autoimmune disorders, and malignancies, where vitamin D dysregulation is implicated. These antibodies are also employed to explore CYP2R1’s interactions with other metabolic pathways and its response to pharmacological agents or environmental factors.
Available as monoclonal or polyclonal variants, CYP2R1 antibodies are validated for techniques like Western blotting, immunohistochemistry, and ELISA. Their specificity and sensitivity make them vital for advancing understanding of vitamin D biology, genetic disorders, and therapeutic strategies targeting CYP2R1 activity.
×
