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产品描述：人甘氨酰-tRNA合成酶(GARS)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的GARS含量。GARS（甘氨酰-tRNA合成酶）是一种重要的蛋白质合成酶，其基因突变可能导致多种神经退行性疾病。研究GARS的靶点，旨在揭示其与疾病的关系，为疾病的治疗提供新思路。研究机制主要涉及GARS在蛋白质合成中的作用，以及其突变如何影响细胞功能。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于细胞生物学、分子机制研究及疾病模型分析，例如探究药物干预、基因编辑或病理条件下GARS的表达调控及其功能变化，为蛋白质合成异常相关研究提供定量工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：GARS1 ELISA Kit; GARSGlycine--tRNA ligase ELISA Kit; EC 6.1.1.14 ELISA Kit; Diadenosine tetraphosphate synthetase ELISA Kit; Ap4A synthetase ELISA Kit; EC 2.7.7.- ELISA Kit; Glycyl-tRNA synthetase ELISA Kit; GlyRS ELISA Kit; Glycyl-tRNA synthetase 1 ELISA Kit
缩写：GARS
Uniprot No.：P41250
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human GARS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 91-99 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 90*98 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-103 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 89-105 | |||||
回收率：
| The recovery of human GARS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 84-92 | ||||
| EDTA plasma (n=4) | 91 | 85-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.1212.0972.1092.00610001.8671.8481.8581.7555001.5381.5271.5331.4302501.1071.1011.1041.0011250.7010.6990.7000.59762.50.4970.4920.4950.39231.250.2830.2750.2790.17600.1050.1010.103 | ||||||
货期：3-5 working days
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