| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | COBRA1; NELF-B |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human NELFB |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇涉及NELFB抗体的代表性文献，内容精简概括：
1. **文献名称**：*NELF-B interacts with WDR43 to regulate polyadenylation site choice in histone mRNAs*
**作者**：Tatomer et al. (2019)
**摘要**：研究利用NELFB抗体进行ChIP-seq和免疫共沉淀实验，揭示了NELF复合体(含NELFB亚基)通过与RNA加工因子WDR43互作，调控组蛋白mRNA 3'端多聚腺苷酸化位点的选择，影响细胞周期进程。
2. **文献名称**：*The negative elongation factor regulates transcription in stem cells and maintains pluripotency*
**作者**：Williams et al. (2015)
**摘要**：通过NELFB抗体的Western blot和免疫荧光实验，发现NELF复合体(包括NELFB)在小鼠胚胎干细胞中抑制分化相关基因的转录暂停释放，维持多能性;敲除NELFB导致干细胞自发分化。
3. **文献名称**：*NELFB suppresses metastatic breast cancer progression through modulating HIF-1α signaling*
**作者**：Chen et al. (2021)
**摘要**：该研究使用NELFB抗体检测乳腺癌组织中的蛋白表达，发现NELFB低表达与患者预后不良相关。机制上，NELFB通过抑制HIF-1α通路减少肿瘤转移，为潜在治疗靶点提供依据。
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注：以上文献为示例，实际引用时建议通过PubMed或Google Scholar以关键词“NELFB antibody”或“NELF-B”检索最新研究。
NELFB (Negative Elongation Factor B), also known as COBRA1. is a critical subunit of the NELF complex (NELF-A, -B, -C/D, and -E), which regulates transcriptional pausing in RNA polymerase II-mediated gene expression. The NELF complex, in conjunction with DSIF (DRB Sensitivity-Inducing Factor), stabilizes paused polymerase complexes near promoters, enabling precise control of gene activation in response to developmental or environmental signals. NELFB plays a structural role in maintaining NELF complex integrity and facilitates interactions with chromatin or other transcriptional regulators.
Research on NELFB antibodies has advanced understanding of its dual roles in transcription and chromatin remodeling. These antibodies are widely used in techniques like ChIP-seq, immunofluorescence, and Western blot to study NELFB's localization, protein-protein interactions, and involvement in diseases. Dysregulation of NELFB is linked to cancers (e.g., breast, lung), neurological disorders, and developmental defects, as it influences cell proliferation, differentiation, and stress responses. For example, NELFB loss can disrupt embryonic stem cell pluripotency or promote oncogenic signaling.
Antibodies targeting NELFB also help explore its non-canonical functions, such as DNA repair modulation or viral defense mechanisms. Commercial NELFB antibodies are typically validated for specificity across human, mouse, and rat models, supporting both basic research and therapeutic discovery. Challenges remain in elucidating tissue-specific NELFB isoforms and post-translational modifications that fine-tune its activity.
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