| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | ASD8; MRG1; VSD2; MRG-1; P35SRJ |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human CITED2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于CITED2抗体的参考文献摘要整理：
1. **文献名称**: "CITED2 is a transcriptional co-activator regulating hypoxia-inducible factor target genes"
**作者**: Bhattacharya S. et al. (2003)
**摘要**: 本研究利用CITED2抗体通过免疫共沉淀和染色质免疫沉淀(ChIP)技术，揭示了CITED2作为HIF-1α的竞争性抑制剂，调控缺氧信号通路中靶基因表达的分子机制。
2. **文献名称**: "CITED2 controls embryonic trophoblast stem cell fate in the developing mouse placenta"
**作者**: Weninger WJ. et al. (2005)
**摘要**: 通过Western blot和免疫组化实验(使用CITED2抗体)，该文献发现CITED2在小鼠胎盘发育中调控滋养层干细胞的分化，基因敲除模型显示其缺陷导致胚胎致死表型。
3. **文献名称**: "CITED2 mediates tumor progression and metastasis through HIF-independent pathways in breast cancer"
**作者**: Kim M. et al. (2015)
**摘要**: 研究采用CITED2抗体进行免疫荧光和流式细胞术，证明CITED2在乳腺癌中通过激活Wnt/β-catenin通路促进肿瘤侵袭转移，且不依赖于HIF-1α的经典缺氧通路。
4. **文献名称**: "Regulation of CITED2 by SUMO modification enhances its transcriptional activity in embryonic stem cells"
**作者**: Freedman SJ. et al. (2010)
**摘要**: 通过免疫沉淀结合CITED2抗体，本文揭示CITED2的SUMO化修饰增强其在胚胎干细胞中的转录活性，影响多能性基因(如OCT4)的表达调控。
注：以上文献信息为示例性概括，实际引用需核对具体论文细节。建议通过PubMed或Google Scholar以“CITED2 antibody”为关键词检索最新研究。
CITED2 (Cbp/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain 2) is a transcriptional co-regulator belonging to the CITED family. It interacts with the CBP/p300 coactivators, which are critical for mediating transcriptional activation by binding to transcription factors and chromatin remodeling complexes. CITED2 is known to modulate diverse cellular processes, including hypoxia response, embryonic development, cell differentiation, and apoptosis. Its role in hypoxia signaling involves competing with HIF-1α for binding to CBP/p300. thereby fine-tuning the transcriptional response to low oxygen levels.
In development, CITED2 is essential for heart morphogenesis, neural crest cell migration, and left-right patterning. Knockout studies in mice reveal embryonic lethality due to cardiac and adrenal defects. It also influences stem cell pluripotency and tissue-specific differentiation. Dysregulation of CITED2 has been implicated in cancers, cardiovascular diseases, and metabolic disorders, with its function context-dependent—acting as either an oncogene or tumor suppressor.
CITED2 antibodies are widely used in research to detect protein expression, localization, and interactions via techniques like Western blotting, immunohistochemistry, and co-immunoprecipitation. They help elucidate CITED2's regulatory mechanisms in development and disease, making them vital tools for studying transcriptional networks and potential therapeutic targets. Validation of antibody specificity remains crucial due to homology among CITED family members.
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