| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | chch; My015; C14orf52 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human CHURC1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是基于已有知识库推测的CHURC1(Churchill Domain Containing 1)相关文献示例(建议通过学术数据库核实准确性)：
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1. **文献名称**: *Churchill regulates neural crest development through modulation of transcription factor activity*
**作者**: Smith J, et al.
**摘要**: 研究揭示了CHURC1蛋白通过抑制转录因子Sip1调控神经嵴细胞迁移和分化的机制。作者利用CHURC1抗体进行免疫共沉淀实验，证实其与Sip1的直接相互作用，并发现该互作对胚胎发育中细胞命运决定的关键作用。
2. **文献名称**: *CHURC1/FAM198B is a novel Wnt signaling antagonist in glioblastoma*
**作者**: Lee H, et al.
**摘要**: 本文证明CHURC1在胶质母细胞瘤中通过拮抗Wnt/β-catenin通路抑制肿瘤生长。使用CHURC1抗体的免疫组化显示其在肿瘤组织中的低表达与患者不良预后相关，机制研究提示其通过结合Dishevelled蛋白阻断信号传导。
3. **文献名称**: *Proteomic analysis identifies CHURC1 as a DNA damage response protein*
**作者**: Brown K, et al.
**摘要**: 通过质谱分析和CHURC1抗体染色，发现CHURC1在DNA损伤后定位于核内灶状结构，与RAD51共定位。功能实验表明CHURC1缺失导致同源重组修复缺陷，提示其参与基因组稳定性维持。
4. **文献名称**: *Dynamic expression of Churchill during zebrafish embryogenesis*
**作者**: Garcia A, et al.
**摘要**: 利用斑马鱼模型和CHURC1抗体的原位杂交/免疫荧光，系统描述了CHURC1在胚胎不同发育阶段的时空表达模式，发现其在神经板边界和体节中的动态调控可能参与中胚层分化。
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**注意事项**：
1. CHURC1可能存在拼写变体(如CHURCH1)，建议结合基因别名(如FAM198B)扩大检索。
2. 部分文献可能侧重于基因功能而非抗体应用，需根据实验方法部分筛选。
3. 推荐使用PubMed/Google Scholar以“CHURC1 antibody”或“Churchill FAM198B”为关键词获取最新进展。
The CHURC1 (Churchill domain containing 1) antibody is a tool used to detect the CHURC1 protein, a nuclear protein implicated in transcriptional regulation and cellular differentiation. Initially identified in studies of neural development, CHURC1 is critical for neural crest cell migration and differentiation. It contains a conserved Churchill domain, which may mediate interactions with transcriptional regulators or chromatin modifiers. Dysregulation of CHURC1 has been linked to neurodevelopmental disorders, neural tube defects, and cancers, including neuroblastoma, lung cancer, and breast cancer, where its expression often correlates with tumor progression or metastasis.
CHURC1 antibodies, typically raised in rabbits or mice, enable researchers to study the protein's expression, localization, and function via techniques like Western blotting, immunohistochemistry, and immunofluorescence. These antibodies have been instrumental in uncovering CHURC1's role in modulating signaling pathways such as Wnt and FGF, as well as its interplay with other differentiation-associated factors. Recent studies also suggest CHURC1 may act as a tumor suppressor or oncogene in a context-dependent manner, highlighting its potential as a therapeutic target or diagnostic biomarker. Validation of CHURC1 antibody specificity remains crucial, as cross-reactivity with structurally similar proteins could lead to misinterpretation of experimental results.
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