| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CEP27; C15orf25; HsT17025 |
| WB Predicted band size | 27 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human HAUS2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于HAUS2抗体的3篇参考文献，按研究主题和抗体应用分类整理：
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1. **文献名称**：*HAUS复合体在纺锤体组装和有丝分裂中的功能研究*
**作者**：Gassmann R, et al.
**摘要**：该研究通过HAUS2抗体进行免疫荧光和Western blot分析，发现HAUS2是HAUS复合体的核心亚基，对纺锤体微管锚定和染色体分离至关重要。敲低HAUS2导致纺锤体结构异常和细胞分裂失败。
2. **文献名称**：*HAUS复合体亚基的分子互作网络分析*
**作者**：Lawo S, et al.
**摘要**：利用HAUS2抗体进行免疫共沉淀实验，揭示了HAUS2与其他亚基(如HAUS1/3)的相互作用，并证明其在维持复合体稳定性中的作用。研究还发现HAUS2缺失会破坏中心体周围微管组织。
3. **文献名称**：*HAUS2在癌症细胞增殖中的调控机制*
**作者**：Zhu H, et al.
**摘要**：通过HAUS2抗体的免疫组化分析，发现HAUS2在多种肿瘤组织中高表达，且其表达水平与患者预后负相关。功能实验表明HAUS2通过调控有丝分裂进程促进癌细胞增殖。
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**备注**：以上文献为示例，实际研究中建议通过PubMed或Web of Science以“HAUS2 antibody”或“HAUS complex”为关键词检索最新论文，重点关注涉及抗体验证(如KO验证、应用场景)的研究。
The HAUS2 antibody is a research tool designed to target the HAUS2 protein, a critical component of the HAUS (HAUS Augmin-like complex) family involved in microtubule organization during cell division. The HAUS complex, comprising eight subunits (HAUS1-HAUS8), facilitates spindle assembly by promoting microtubule branching and nucleation, ensuring proper mitotic spindle formation and chromosomal segregation. HAUS2. also known as DGT6. plays a key role in recruiting γ-tubulin ring complexes to spindle microtubules, enhancing their stability and density. This function is evolutionarily conserved across eukaryotes, reflecting its fundamental role in cell cycle regulation.
Antibodies against HAUS2 are widely used in molecular biology to study its expression, localization, and interactions. They enable techniques like Western blotting, immunofluorescence, and co-immunoprecipitation, aiding in the investigation of mitotic mechanisms, spindle dynamics, and cell division defects. HAUS2 dysregulation has been linked to mitotic errors, genomic instability, and diseases such as cancer, making its antibody valuable for both basic research and clinical studies.
Most HAUS2 antibodies are raised in rabbits or mice using immunogenic peptide sequences specific to conserved regions of the protein. Validation typically includes knockout cell lines or siRNA knockdown to confirm specificity. These reagents are essential for elucidating HAUS2's contributions to cellular homeostasis and its potential as a therapeutic target in pathologies linked to mitotic dysfunction.
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