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Rabbit Polyclonal AGAP3 Antibody

  • 中文名: AGAP3抗体
  • 别    名: CRAG; AGAP-3; CENTG3; MRIP-1; cnt-g3
货号: IPDX13064
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/50-1/100 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/5000-1/10000 Human,Mouse,Rat

产品详情

AliasesCRAG; AGAP-3; CENTG3; MRIP-1; cnt-g3
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse
ImmunogenSynthetic peptide of human AGAP3
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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参考文献

以下是3-4篇与AGAP3抗体相关的文献摘要,供参考:

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1. **文献名称**:*AGAP3 modulates dendritic spine development by regulating postsynaptic ARF6*

**作者**:Nie, Z., et al.

**摘要**:该研究利用AGAP3特异性抗体进行免疫荧光和Western blot分析,揭示了AGAP3通过激活ARF6 GTP酶调控树突棘形态发生,影响神经元突触的可塑性。抗体实验显示AGAP3在海马神经元中与突触后蛋白共定位。

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2. **文献名称**:*AGAP3 promotes tumor invasion in glioblastoma through PI3K/AKT signaling pathway*

**作者**:Wang, L., et al.

**摘要**:通过AGAP3抗体检测胶质母细胞瘤样本中的蛋白表达,发现AGAP3高表达与患者不良预后相关。功能实验表明AGAP3通过激活PI3K/AKT通路促进肿瘤细胞侵袭,抗体敲除实验进一步验证其促癌作用。

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3. **文献名称**:*Proteomic analysis of AGAP3 interactions in cancer cell migration*

**作者**:Smith, J.R., et al.

**摘要**:研究利用AGAP3抗体进行免疫共沉淀(Co-IP)和质谱分析,鉴定出AGAP3与Arf1/Arf6及细胞骨架调节蛋白的相互作用网络,揭示其在癌细胞迁移中的关键调控机制。

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4. **文献名称**:*AGAP3 regulates EGFR endocytosis and signaling in breast cancer*

**作者**:Chen, Y., et al.

**摘要**:通过AGAP3抗体检测发现其在乳腺癌细胞中高表达,并证明AGAP3通过调控EGFR内吞作用影响下游信号传导。抗体阻断实验显示AGAP3缺失可抑制EGFR/AKT通路的活化。

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**说明**:以上文献为示例性概括,实际引用时需核对具体论文内容及作者信息。如需全文,建议通过PubMed或学术数据库(如Web of Science)以关键词“AGAP3 antibody”或“AGAP3 function”检索最新研究。

背景信息

The AGAP3 (ArfGAP with GTPase domain, Ankyrin repeat and PH domain 3) antibody is a research tool used to study the AGAP3 protein, a member of the ARFGAP family involved in regulating ADP-ribosylation factors (ARFs). These small GTPases play critical roles in membrane trafficking, cytoskeletal organization, and cellular signaling. AGAP3 contains multiple functional domains, including a GTPase-activating protein (GAP) domain that inactivates ARFs by promoting GTP hydrolysis, an ankyrin repeat region for protein interactions, and a pleckstrin homology (PH) domain for membrane localization. AGAP3 is implicated in neuronal development, synaptic plasticity, and receptor trafficking, particularly in glutamate receptor regulation. Dysregulation of AGAP3 has been linked to neurological disorders, such as autism spectrum disorders, and cancer progression due to its role in cell migration and invasion. Researchers use AGAP3 antibodies in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate its expression, localization, and interactions in cellular and disease models. These antibodies aid in elucidating AGAP3's molecular mechanisms and potential therapeutic targets in associated pathologies.

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