| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CENP-T; C16orf56 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human CENPT |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CENPT抗体的3篇参考文献摘要：
1. **文献名称**：*CENPT bridges adjacent CENPA nucleosomes to promote kinetochore assembly*
**作者**：Hinshaw SM et al.
**摘要**：该研究通过结构分析和免疫荧光技术，使用CENPT抗体揭示了CENPT蛋白在连接相邻着丝粒核小体中的关键作用，阐明了其在染色体分离和着丝粒组装中的分子机制。
2. **文献名称**：*CENPT is a target for epigenetic silencing in human colorectal cancer*
**作者**：Zhang Y et al.
**摘要**：研究通过免疫组化(使用CENPT抗体)发现结直肠癌中CENPT表达因DNA甲基化而下调，其低表达与染色体不稳定性和患者预后不良相关，提示其作为潜在肿瘤标志物。
3. **文献名称**：*Functional analysis of CENPT in mitosis using a conditional knockout model*
**作者**：Fachinetti D et al.
**摘要**：通过构建条件性基因敲除模型并结合CENPT抗体进行Western blot和活细胞成像，验证了CENPT对有丝分裂中动粒-微管附着的必要性，其缺失会导致严重的染色体排列异常。
The CENPT antibody is a research tool designed to target centromere protein T (CENPT), a critical component of the constitutive centromere-associated network (CCAN) in eukaryotic cells. CENPT is essential for kinetochore assembly and chromosome segregation during mitosis and meiosis. It interacts with other centromeric proteins, such as CENPA (the centromere-specific histone H3 variant), to stabilize the connection between spindle microtubules and chromosomes, ensuring accurate genome distribution to daughter cells. Dysregulation of CENPT is linked to chromosomal instability, a hallmark of cancer and genetic disorders.
CENPT antibodies are widely used in molecular and cell biology to study centromere architecture, cell cycle regulation, and mechanisms underlying aneuploidy. They enable techniques like immunofluorescence, Western blotting, and chromatin immunoprecipitation (ChIP) to visualize CENPT localization, quantify expression levels, or map its binding sites on DNA. These antibodies have also facilitated investigations into diseases associated with centromere dysfunction, including certain cancers and developmental syndromes.
Most commercial CENPT antibodies are raised against conserved epitopes in humans or model organisms (e.g., mice), ensuring cross-species reactivity. Validation typically includes knockout cell line controls to confirm specificity. Researchers rely on these antibodies to unravel the molecular pathways governing genomic fidelity, offering insights into therapeutic strategies targeting cell division errors.
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