| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CDC14B3; Cdc14B1; Cdc14B2; hCDC14B |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human CDC14B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CDC14B抗体的3篇参考文献的模拟示例(注：以下文献信息为假设性概括，实际文献需通过学术数据库核实)：
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1. **文献名称**: *CDC14B regulates cell cycle progression through dephosphorylation of cyclin-dependent kinase substrates*
**作者**: Gray CH, et al.
**摘要**: 本研究利用CDC14B特异性抗体，通过免疫印迹和免疫荧光技术，揭示了CDC14B磷酸酶在G2/M期调控中的作用。实验表明，CDC14B通过去磷酸化CDK1底物促进有丝分裂退出，并在DNA损伤修复中起关键作用。
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2. **文献名称**: *Localization and functional analysis of CDC14B in mammalian cell division*
**作者**: Mocciaro A, et al.
**摘要**: 通过使用针对CDC14B的多克隆抗体，作者发现该蛋白定位于中心体和中期纺锤体。功能研究表明，CDC14B缺失导致染色体分离异常，提示其参与维持基因组稳定性。
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3. **文献名称**: *CDC14B expression correlates with tumor progression in colorectal cancer*
**作者**: Li Y, et al.
**摘要**: 研究通过免疫组化(IHC)分析CDC14B抗体在结直肠癌组织中的表达，发现其低表达与患者预后不良相关，提示CDC14B可能作为潜在的肿瘤抑制因子。
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如需真实文献，建议在PubMed或Google Scholar中搜索关键词“CDC14B antibody”并筛选近年研究。
The CDC14B antibody is a crucial tool for studying the CDC14B phosphatase, a member of the conserved CDC14 family involved in cell cycle regulation. Originally characterized in yeast for its role in mitotic exit and DNA damage response, mammalian CDC14B has more complex functions, including modulating G2/M transition, centrosome maturation, and chromosome segregation. It counteracts cyclin-dependent kinase (CDK) activity by dephosphoryulating specific substrates, ensuring proper cell cycle progression and genomic stability. Dysregulation of CDC14B is linked to diseases such as cancer, where its altered expression may contribute to tumorigenesis by disrupting cell cycle checkpoints or promoting aneuploidy.
CDC14B antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to detect protein expression levels, subcellular localization, and post-translational modifications. These antibodies help elucidate CDC14B's dynamic roles in cellular processes, such as its nucleolar sequestration during interphase and release during mitosis. Researchers also employ them to investigate CDC14B's interaction networks, including its association with regulatory proteins like CKAP2 or components of the anaphase-promoting complex (APC/C). Commercial antibodies are typically validated for specificity in human, mouse, or rat models, with applications spanning basic research and clinical studies exploring CDC14B's potential as a therapeutic target or biomarker in cancer and developmental disorders.
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