| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | cdc14; hCDC14; DFNB105 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human CDC14A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是假设的关于CDC14A抗体的参考文献示例(实际文献需通过学术数据库查询)：
1. **"CDC14A phosphatase regulates ciliogenesis through dephosphorylation of TTBK2 kinase"**
*Authors: Yuan et al. (2015)*
**摘要**：研究利用CDC14A特异性抗体，发现其通过去磷酸化TTBK2调控纤毛形成，揭示了CDC14A在精子发生中的关键作用。
2. **"Cell cycle-dependent regulation of DNA damage response by CDC14A"**
*Authors: Smith et al. (2018)*
**摘要**：通过免疫荧光和Western blot(使用CDC14A抗体)，证明CDC14A在DNA损伤修复中协调ATR-Chk1信号通路，影响细胞周期检查点。
3. **"CDC14A expression correlates with poor prognosis in colorectal cancer"**
*Authors: Li et al. (2020)*
**摘要**：采用CDC14A抗体进行免疫组化分析，发现其高表达与结直肠癌患者生存率下降相关，提示其作为潜在预后标志物。
4. **"CDC14A knockout mice exhibit neural tube defects via dysregulated Wnt signaling"**
*Authors: Johnson et al. (2019)*
**摘要**：通过抗体检测CDC14A在胚胎发育中的表达，揭示其缺失导致Wnt通路异常，引发神经管闭合缺陷。
*注：以上为假设文献，实际研究请查询PubMed或Web of Science等数据库。*
The CDC14A antibody is a crucial tool for studying the dual-specificity phosphatase CDC14A, a key regulator of cell cycle progression. CDC14A, part of the conserved CDC14 phosphatase family, plays pivotal roles in mitotic exit, centrosome maturation, and DNA damage response by dephosphorylating cell cycle-related substrates such as CDK1 and certain E3 ubiquitin ligases. It counteracts cyclin-dependent kinase (CDK) activity to ensure orderly transitions between cell cycle phases. Research links CDC14A dysfunction to genomic instability, developmental disorders, and cancers, making it a focus in oncology and cell biology studies.
The CDC14A antibody is widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to detect protein expression, subcellular localization (e.g., nucleolar distribution during interphase), and interaction partners. Its specificity varies across commercial clones, requiring validation via knockout cell lines or siRNA knockdown controls. Studies employing this antibody have revealed CDC14A's context-dependent roles—while essential in some cancers, its loss may paradoxically drive malignancy in others. Recent work also implicates CDC14A in cilia disassembly and neurological development. Researchers prioritize antibodies validated in peer-reviewed publications to ensure reproducibility, particularly given CDC14A's overlapping functions with its paralog CDC14B. Proper controls are critical due to potential cross-reactivity or batch variability.
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