首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人FMS样酪氨酸激酶3(Flt3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FLT3含量。FLT3 是一种受体酪氨酸激酶，在造血干细胞的生长、分化等过程中起关键作用。其异常激活与血液系统恶性肿瘤密切相关，如急性髓系白血病。当前对它的研究主要围绕其信号通路机制，以探索阻断异常信号、抑制肿瘤细胞增殖和存活的有效方法。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于血液学研究、肿瘤机制探索及药物开发等科研场景，例如通过检测白血病模型动物血清中Flt3水平评估靶向治疗效果，或分析实体瘤组织匀浆中Flt3表达特征以探究其与肿瘤微环境的关联。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CD 135 ELISA Kit; CD135 ELISA Kit; CD135 antigen ELISA Kit; Fetal liver kinase 2 ELISA Kit; FL cytokine receptor ELISA Kit; Flk 2 ELISA Kit; Flk2 ELISA Kit; Flt 3 ELISA Kit; FLT-3 ELISA Kit; Flt3 ELISA Kit; FLT3_HUMAN ELISA Kit; FMS like tyrosine kinase 3 ELISA Kit; Fms related tyrosine kinase 3 ELISA Kit; Fms-like tyrosine kinase 3 ELISA Kit; Growth factor receptor tyrosine kinase type III ELISA Kit; Ly-72 ELISA Kit; OTTHUMP0000004234 ELISA Kit; Receptor type tyrosine protein kinase FLT3 ELISA Kit; Stem cell tyrosine kinase 1 ELISA Kit; Stk 1 ELISA Kit; STK-1 ELISA Kit; Stk1 ELISA Kit; Tyrosine protein kinase receptor FLT3 ELISA Kit; Tyrosine-protein kinase receptor FLT3 ELISA Kit
缩写：FLT3
Uniprot No.：P36888
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human Flt3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 90 | |||||
| Range % | 85-95 | ||||||
| 1:2 | Average % | 92 | |||||
| Range % | 88-96 | ||||||
| 1:4 | Average % | 87 | |||||
| Range % | 82-91 | ||||||
| 1:8 | Average % | 95 | |||||
| Range % | 90-100 | ||||||
回收率：
| The recovery of human Flt3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 89 | 85-93 | |||||
| EDTA plasma (n=4) | 103 | 98-110 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected40002.4012.2382.3202.14320001.6851.7961.7411.56410001.3161.2751.2961.1195000.8740.9020.8880.7112500.5930.5690.5810.4041250.4020.4110.4070.23062.50.2690.2570.2630.08600.1800.1730.177 | |||||||
货期：3-5 working days
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