Rat basic fibroblast growth factor,bFGF ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E08002r

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
大鼠碱性成纤维生长因子(bFGF)酶联免疫试剂盒检测血清、血浆、组织匀浆样本中的FGF2含量。FGF2即成纤维细胞生长因子 2，在细胞生长、增殖和分化等过程中发挥关键作用。研究其机制发现，它通过与特定受体结合，激活下游信号通路，影响细胞行为。在肿瘤、心血管疾病等领域有潜在应用，可作为治疗靶点研究干预策略。试剂盒检测范围为0.312 ng/ml-20 ng/ml，该产品适用于科研领域探究脑损伤修复、肿瘤微环境或心血管疾病模型中bFGF的表达动态，可为体外实验提供可靠的定量分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Fgf2 ELISA Kit; Fgf-2Fibroblast growth factor 2 ELISA Kit; FGF-2 ELISA Kit; Basic fibroblast growth factor ELISA Kit; bFGF ELISA Kit; Heparin-binding growth factor 2 ELISA Kit; HBGF-2 ELISA Kit
缩写：
FGF2
Uniprot No.：
P13109
种属：
Rattus norvegicus (Rat)
样本类型：
serum, plasma, tissue homogenates
检测范围：
0.312 ng/ml -20 ng/ml
灵敏度：
0.078 ng/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cardiovascular
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat bFGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	90
	Range %	84-98
1:2	Average %	94
	Range %	91-102
1:4	Average %	100
	Range %	92-109
1:8	Average %	93
	Range %	86-98

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
20	2.558	2.634	2.596	2.405
10	1.508	1.567	1.537	1.346
5	0.924	0.928	0.926	0.735
2.5	0.556	0.542	0.549	0.358
1.25	0.417	0.421	0.419	0.228
0.625	0.337	0.348	0.343	0.152
0.312	0.272	0.284	0.278	0.087
0	0.187	0.195	0.191