首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠脂肪酸合成酶(FAS)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FASN含量。FASN 全称脂肪酸合酶，它在脂肪酸合成中起关键作用。在肿瘤细胞中，FASN 常高表达以满足其快速增殖的代谢需求。研究机制方面，主要通过抑制 FASN 活性来阻断肿瘤细胞脂肪酸合成，干扰其细胞膜形成、能量代谢等过程，成为潜在抗癌靶点。试剂盒检测范围为1.56 ng/mL-100 ng/mL，适用于探索代谢性疾病发生机制、评估营养干预对脂代谢的影响，或作为药物开发中靶向FAS通路的生物标志物分析工具；支持科研人员在肝脏代谢研究、脂肪组织功能评估或能量代谢相关动物模型实验中快速获取稳定可靠的FAS浓度数据，为深入揭示脂质合成调控网络提供实验支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FasnFatty acid synthase ELISA kit; EC 2.3.1.85) [Includes: [Acyl-carrier-protein] S-acetyltransferase ELISA kit; EC 2.3.1.38); [Acyl-carrier-protein] S-malonyltransferase ELISA kit; EC 2.3.1.39); 3-oxoacyl-[acyl-carrier-protein] synthase ELISA kit; EC 2.3.1.41); 3-oxoacyl-[acyl-carrier-protein] reductase ELISA kit; EC 1.1.1.100); 3-hydroxyacyl-[acyl-carrier-protein] dehydratase ELISA kit; EC 4.2.1.59); Enoyl-[acyl-carrier-protein] reductase ELISA kit; EC 1.3.1.39); Oleoyl-[acyl-carrier-protein] hydrolase ELISA kit; EC 3.1.2.14)] ELISA kit
缩写：FASN
Uniprot No.：P12785
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat FAS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 88-99 | |||||
| 1:4 | Average % | 88 | ||||
| Range % | 84-92 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-100 | |||||
回收率：
| The recovery of rat FAS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 100 | 96-108 | ||||
| EDTA plasma (n=4) | 97 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected1002.5722.4272.5002.371501.8371.9021.8701.741251.3531.3081.3311.20212.50.7750.8190.7970.6686.250.5120.5160.5140.3853.120.3050.2940.3000.1711.560.2450.2510.2480.11900.1310.1270.129 | ||||||
货期：3-5 working days
×
