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产品描述:人膜外核苷酸焦磷酸酶/磷酸二酯酶家族成员3(ENPP3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆、细胞裂解物样本中的ENPP3含量。ENPP3 作为一个靶点,在众多疾病研究中备受关注。其背景与细胞信号传导、肿瘤微环境等密切相关。研究机制方面,它参与调节细胞内外的核苷酸代谢,还可能影响肿瘤细胞的增殖、迁移等过程,为相关疾病的治疗提供了重要潜在靶点。试剂盒检测范围为0.156 ng/mL-10 ng/mL,适用于基础科研中ENPP3蛋白表达的动态监测,如探究其在肿瘤微环境、免疫调节或代谢性疾病模型中的功能机制,也可用于细胞实验、动物模型或生物样本库中ENPP3水平的系统性比较研究,为分子机制探索提供可靠数据支持。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:Alkaline phosphodiesterase I ELISA Kit; ARHR2 ELISA Kit; B10 ELISA Kit; CD203c ELISA Kit; CD203c antigen ELISA Kit; dJ1005H11.3 (phosphodiesterase I/nucleotide pyrophosphatase 3) ELISA Kit; dJ914N13.3 (phosphodiesterase I/nucleotide pyrophosphatase 3) ELISA Kit; E NPP 3 ELISA Kit; E-NPP 3 ELISA Kit; Ectonucleotide pyrophosphatase/phosphodiesterase 3 ELISA Kit; Ectonucleotide pyrophosphatase/phosphodiesterase family member 3 ELISA Kit; ENPP3 ELISA Kit; ENPP3_HUMAN ELISA Kit; gp130RB13 6 ELISA Kit; gp130RB136 ELISA Kit; M6S1 ELISA Kit; NPP1 ELISA Kit; NPP3 ELISA Kit; NPPase ELISA Kit; NPPS ELISA Kit; Nucleotide pyrophosphatase ELISA Kit; PC 1 ELISA Kit; PC-1 ELISA Kit; PCA1 ELISA Kit; PD Ibeta ELISA Kit; PD-Ibeta ELISA Kit; PDNP1 ELISA Kit; PDNP3 ELISA Kit; Phosphodiesterase I beta ELISA Kit; Phosphodiesterase I/nucleotide pyrophosphatase 3 ELISA Kit; phosphodiesterase i/nucleotide pyrophosphatase beta ELISA Kit
缩写:ENPP3
Uniprot No.:O14638
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates, cell lysates
检测范围:0.156 ng/mL-10 ng/mL
灵敏度:0.039 ng/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Epigenetics and Nuclear Signaling
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human ENPP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:1 | Average % | 97 | |||||
Range % | 93-101 | ||||||
1:2 | Average % | 99 | |||||
Range % | 92-105 | ||||||
1:4 | Average % | 89 | |||||
Range % | 83-94 | ||||||
1:8 | Average % | 91 | |||||
Range % | 87-96 |
回收率:
The recovery of human ENPP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 104 | 98-109 | |||||
EDTA plasma (n=4) | 85 | 80-90 | |||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
ng/mlOD1OD2AverageCorrected102.7552.8582.8072.69152.2122.2212.2172.1012.51.5371.4771.5071.3911.250.9460.9540.9500.8340.6250.4890.4730.4810.3650.3120.2890.2740.2820.1660.1560.2100.2180.2140.09800.1150.1160.116 |
货期:3-5 working days
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