首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠α烯醇化酶(α-enolase)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ENO1含量。ENO1是一个重要靶点。它参与糖酵解等关键代谢过程。研究发现，ENO1异常表达与肿瘤发生发展密切相关，其可能通过影响能量代谢、细胞增殖与凋亡等机制来发挥作用，正成为肿瘤治疗等领域有潜力的研究方向。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于科研场景下对大鼠模型代谢通路异常、疾病机制探索或药物干预效果评估等实验需求，为生命科学基础研究提供可靠工具。 其在代谢性疾病、肿瘤微环境及炎症反应等研究领域具有重要价值。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Eno1 ELISA Kit; Eno-1Alpha-enolase ELISA Kit; EC 4.2.1.11 ELISA Kit; 2-phospho-D-glycerate hydro-lyase ELISA Kit; Enolase 1 ELISA Kit; Non-neural enolase ELISA Kit; NNE ELISA Kit
缩写：ENO1
Uniprot No.：P04764
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat α-enolase in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 93 | |||||
| Range % | 88-97 | ||||||
| 1:2 | Average % | 102 | |||||
| Range % | 97-107 | ||||||
| 1:4 | Average % | 101 | |||||
| Range % | 95-105 | ||||||
| 1:8 | Average % | 94 | |||||
| Range % | 91-98 | ||||||
回收率：
| The recovery of rat α-enolase spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 105 | 99-108 | |||||
| EDTA plasma (n=4) | 87 | 84-92 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected402.1912.2752.2332.111201.4481.5381.4931.371100.9210.9630.9420.82050.5630.5720.5680.4462.50.3340.3410.3380.2161.250.2310.2450.2380.1160.6250.1730.1790.1760.05400.1210.1220.122 | |||||||
货期：3-5 working days
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