Rat dipeptidyl peptldase Ⅳ,DPPⅣ ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E08519r

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
大鼠二肽基肽酶Ⅳ(DPPⅣ)酶联免疫试剂盒双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的DPP4含量。DPP4即二肽基肽酶4，是一种丝氨酸蛋白酶。它参与多种生理过程，在糖代谢调节等方面有重要作用。研究机制表明，DPP4可降解胰高血糖素样肽 -1等肠促胰素，降低其活性。通过抑制DPP4，能提高肠促胰素水平，发挥降血糖等功效。试剂盒检测范围为0.78 ng/ml- 50 ng/mL，该产品适用于体外实验中对生物样本中DPPⅣ含量的动态监测，如药物干预后酶活性变化研究、疾病模型构建中生物标志物分析等科研场景，为代谢机制探索及潜在治疗靶点验证提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Dpp4; Cd26; Dipeptidyl peptidase 4; Bile canaliculus domain-specific membrane glycoprotein; Dipeptidyl peptidase IV; DPP IV; GP110 glycoprotein; T-cell activation antigen CD26; CD antigen CD26
缩写：
DPP4
Uniprot No.：
P14740
种属：
Rattus norvegicus (Rat)
样本类型：
serum, plasma, tissue homogenates
检测范围：
0.78 ng/mL - 50 ng/mL
灵敏度：
0.195 ng/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat DPPⅣ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:2	Average %	88
	Range %	80-92
1:4	Average %	93
	Range %	91-95
1:8	Average %	98
	Range %	94-103
1:16	Average %	93
	Range %	86-98

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
50	2.297	2.164	2.231	2.053
25	1.263	1.327	1.295	1.117
12.5	0.786	0.821	0.803	0.625
6.25	0.523	0.519	0.521	0.343
3.12	0.302	0.321	0.312	0.133
1.56	0.245	0.253	0.249	0.071
0.78	0.214	0.204	0.209	0.031
0	0.181	0.175	0.178