首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠双氢睾酮(DHT)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的DHT含量。DHT（分布式哈希表）是一种去中心化网络技术，通过将资源信息分散存储在多个节点上，实现高效组织和定位。每个节点负责存储部分资源信息和成员联系信息，通过哈希算法和节点ID关联，实现资源查找和节点通信。试剂盒检测范围为10 pg/mL-2000 pg/mL，科研人员可将其应用于内分泌调控研究、雄性生殖生物学实验、药物开发中激素代谢评估，或探究前列腺疾病、脱发等病理模型的激素水平变化本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：DHT
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：10 pg/mL-2000 pg/mL
灵敏度：5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat DHT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 102 | ||||
| Range % | 98-106 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 89-97 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 89-99 | |||||
| 1:8 | Average % | 102 | ||||
| Range % | 98-106 | |||||
回收率：
| The recovery of rat DHT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-95 | ||||
| EDTA plasma (n=4) | 102 | 97-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 20000.1560.1600.158 5000.3120.3290.321 1600.4980.5280.513 400.8180.7870.803 101.3161.2781.297 01.9601.9541.957 | ||||||
本试剂盒所含材料：
A 96-well Assay plate -- The 96-well plate has been pre-coated with goat-anti-rabbit DHT antibody.
Standard 6 x 0.5 ml -- Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
Anti-mouse DHT Antibody 1 x 6 ml -- Act as the detection antibody.
HRP-conjugate 1 x 6 ml -- Bind to the DHT antibody, and HRP catalyzes the TMB to elicit a chromogenic reaction.
Wash Buffer (20 x concentrate) 1 x 15 ml -- Wash away unbound or free substances.
Substrate A 1 x 7 ml -- Mix with substrate B, and the TMB is catalyzed by HRP to develop color.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
An incubator that can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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