首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人DEK蛋白(DEK)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的DEK含量。DEK是一种重要靶点。它在细胞增殖、分化等过程有参与，与多种生理病理机制相关。研究发现，DEK在肿瘤发生发展中发挥作用，可能影响肿瘤细胞生长、侵袭与转移。目前对其深入研究，以揭示机制，为相关疾病治疗提供新方向。试剂盒检测范围为23.44 pg/mL-1500 pg/mL，适用于基础科研中探究DEK蛋白在疾病模型、细胞信号通路或分子机制中的表达变化，例如肿瘤微环境研究、炎症反应分析或药物干预效果评估等场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：D6S231E ELISA Kit; Dek ELISA Kit; DEK gene ELISA Kit; DEK oncogene ELISA Kit; DEK oncogene DNA binding ELISA Kit; DEK_HUMAN ELISA Kit; Protein DEK ELISA Kit
缩写：DEK
Uniprot No.：P35659
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：23.44 pg/mL-1500 pg/mL
灵敏度：5.86 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human DEK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 92-101 | |||||
| 1:2 | Average % | 106 | ||||
| Range % | 101-113 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 89-98 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 85-94 | |||||
回收率：
| The recovery of human DEK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-100 | ||||
| EDTA plasma (n=4) | 88 | 84-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected15002.3942.4292.4122.3087502.0021.9141.9581.8543751.4851.5171.5011.397187.50.9610.9160.9390.83593.750.6230.6010.6120.50846.880.3350.3540.3450.24123.440.2260.2360.2310.12700.1020.1050.104 | ||||||
货期：3-5 working days
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