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产品描述：人β-防御素3酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液、组织培养上清液、组织匀浆样本中的DEFB103A含量。DEFB103A，也称为beta Defensin 3，是一种由中性粒细胞产生的抗菌肽，属于β-防御素家族。该蛋白在免疫系统中发挥重要作用，具有抗菌、免疫调节和保护作用。其机制可能涉及抑制肿瘤生长和增殖，促进抗肿瘤免疫反应。试剂盒检测范围为125 pg/mL-8000 pg/mL，可满足科研实验中定量分析hBD-3在疾病模型、免疫调控机制或药物干预研究中的动态变化需求，为探索其在感染、自身免疫性疾病或肿瘤微环境中的作用提供可靠工具；适用于体外样本的批量检测，为研究人员评估hBD-3的生物学功能及相关通路研究提供高效解决方案本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BD 3 ELISA Kit; BD-3 ELISA Kit; BD3 ELISA Kit; beta 103 ELISA Kit; Beta defensin 103A ELISA Kit; Beta defensin 103A precursor ELISA Kit; Beta defensin 3 ELISA Kit; Beta-defensin 103 ELISA Kit; Beta-defensin 3 ELISA Kit; D103A_HUMAN ELISA Kit; DEF B3 ELISA Kit; DEFB 103 ELISA Kit; DEFB 103A ELISA Kit; DEFB 3 ELISA Kit; DEFB-3 ELISA Kit; DEFB103 ELISA Kit; DEFB103A ELISA Kit; DEFB103B ELISA Kit; DEFB3 ELISA Kit; Defensin ELISA Kit; Defensin beta 103A ELISA Kit; Defensin beta 3 ELISA Kit; Defensin like protein ELISA Kit; Defensin-like protein ELISA Kit; HBD 3 ELISA Kit; hBD-3 ELISA Kit; HBD3 ELISA Kit; HBP 3 ELISA Kit; HBP3 ELISA Kit; mBD3 ELISA Kit
缩写：DEFB103A
Uniprot No.：P81534
种属：Homo sapiens (Human)
样本类型：serum, plasma, urine, cell culture supernates, tissue homogenates
检测范围：125 pg/mL-8000 pg/mL
灵敏度：31.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human β-defensins 3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 91-101 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 92-104 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 86-94 | |||||
回收率：
| The recovery of human β-defensins 3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 87 | 82-94 | ||||
| EDTA plasma (n=4) | 102 | 98-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected80002.3392.5082.4242.27040001.8331.8691.8511.69720001.1851.1981.1921.03810000.6990.6870.6930.5395000.3750.3920.3840.2302500.2940.2810.2880.1341250.2080.2110.2100.05600.1530.1550.154 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human BD3 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled BD3 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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