首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人防御素5(DEFA5)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的DEFA5含量。DEFA5，即人α防御素5，是一种参与宿主防御的抗菌和细胞毒性肽，主要在回肠潘氏细胞中表达。其通过选择性杀死细菌病原体来控制肠道微生物群的组成，并通过分子内二硫桥连接的小型阳离子肽结构发挥抗菌作用。研究显示，DEFA5在肠道微生物群平衡和免疫保护中扮演重要角色。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于肠道炎症机制、病原体感染免疫应答、抗菌肽功能研究等科研领域，兼容多种生物样本类型，为体外研究DEFA5在免疫调节、微生物互作或疾病模型中的表达水平提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：alpha 5 ELISA Kit; DEF5 ELISA Kit; DEF5_HUMAN ELISA Kit; Defa ELISA Kit; Defa29 ELISA Kit; DEFA5 ELISA Kit; Defcr29 ELISA Kit; Defcr5 ELISA Kit; Defensin 5 ELISA Kit; Defensin alpha 5 ELISA Kit; Defensin alpha 5 Paneth cell specific ELISA Kit; Defensin alpha 5 preproprotein ELISA Kit; Defensin ELISA Kit; Enteric defensin ELISA Kit; HD 5 ELISA Kit; HD5 ELISA Kit; HD5(20-94) ELISA Kit; HD5(63-94) ELISA Kit; MGC129728 ELISA Kit; RD 5 ELISA Kit
缩写：DEFA5
Uniprot No.：Q01523
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Microbiology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human DEFA5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 82-92 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 92-102 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 87-98 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-100 | |||||
回收率：
| The recovery of human DEFA5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 95 | 90-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 402.5182.3862.4522.357 202.0932.0512.0721.977 101.6961.6041.6501.555 51.1311.0571.0940.999 2.50.7650.7070.7360.641 1.250.4330.4290.4310.336 0.6250.2140.1970.2060.111 00.0990.0910.095 | ||||||
货期：3-5 working days
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