首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠二胺氧化酶(DAO)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的DAO含量。DAO即去中心化自治组织，背景是随着区块链技术发展应运而生，旨在以智能合约实现组织的自治管理。其研究机制围绕如何通过代码和规则确保组织运作，减少人为干预，实现公平、透明决策，在金融、社区治理等领域有广泛应用前景。试剂盒检测范围为0.78 mIU/mL-50 mIU/mL，适用于肠道疾病模型研究、肠屏障功能评估、炎症机制探索等科研领域，例如通过检测实验动物血清或组织样本中DAO水平，研究肠道损伤修复、药物干预效果或病理生理过程。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Dao ELISA Kit; Dao1D-amino-acid oxidase ELISA Kit; DAAO ELISA Kit; DAMOX ELISA Kit; DAO ELISA Kit; EC 1.4.3.3 ELISA Kit
缩写：DAO
Uniprot No.：O35078
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.78 mIU/mL-50 mIU/mL
灵敏度：0.195 mIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat DAO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 85 | ||||
| Range % | 80-91 | |||||
| 1:200 | Average % | 93 | ||||
| Range % | 89-99 | |||||
| 1:400 | Average % | 87 | ||||
| Range % | 82-95 | |||||
| 1:800 | Average % | 101 | ||||
| Range % | 98-104 | |||||
回收率：
| The recovery of rat DAO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 85 | 81-92 | ||||
| EDTA plasma (n=4) | 90 | 85-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mIU/ml.OD1OD2AverageCorrected502.6342.6722.6532.459252.2672.2982.2832.08912.51.7061.7651.7361.5426.251.1141.1561.1350.9413.120.6230.6720.6480.4541.560.4920.4800.4860.2920.780.3070.3220.3150.12100.1930.1950.194 | ||||||
货期：3-5 working days
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