首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠D二聚体(D2D)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、细胞裂解物样本中的D2D含量。大鼠D二聚体（D2D）是纤维蛋白单体交联后经纤溶酶水解产生的特异性降解产物，是纤溶过程的标记物。其在血栓形成和溶解过程中扮演重要角色，其检测对于评估血栓性疾病具有重要意义，可用于临床诊断和科研研究。试剂盒检测范围为7.8 ng/mL-500 ng/mL，本产品在凝血功能研究、血栓相关疾病模型建立及药物作用机制探索中具有重要科研价值，适用于心血管疾病研究、抗凝药物开发、炎症反应评估等科研场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：D2D
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates, cell lysates
检测范围：7.8 ng/mL-500 ng/mL
灵敏度：1.95 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat D2D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 89-99 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 86-94 | |||||
| 1:8 | Average % | 102 | ||||
| Range % | 98-106 | |||||
回收率：
| The recovery of rat D2D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 85 | 81-92 | ||||
| EDTA plasma (n=4) | 89 | 85-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected5002.4652.3742.4202.2382501.6611.7611.7111.5291251.0521.1451.0990.91762.50.6830.7020.6930.51131.20.4970.4590.4780.29615.60.3210.3190.3200.1387.80.2580.2470.2530.07100.1840.1790.182 | ||||||
货期：3-5 working days
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