首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠CYR61蛋白(CYR61)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CYR61含量。CYR61是一种细胞外基质相关蛋白，在细胞增殖、分化、黏附等多种生理过程中发挥重要作用。研究发现，它与肿瘤的发生、发展、转移密切相关，其机制可能涉及调节细胞信号通路，影响肿瘤微环境等，是潜在的肿瘤治疗靶点。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于心血管疾病模型、肿瘤机制研究、炎症反应评估或再生医学等领域的基础科研，通过检测体液或组织样本中CYR61的动态变化，为探索其分子功能及信号通路提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ccn1 ELISA kit; Cyr61 ELISA kit; Igfbp10CCN family member 1 ELISA kit; Cellular communication network factor 1 ELISA kit; Cysteine-rich angiogenic inducer 61 ELISA kit; Insulin-like growth factor-binding protein 10 ELISA kit; IBP-10 ELISA kit; IGF-binding protein 10 ELISA kit; IGFBP-10 ELISA kit; Protein CYR61 ELISA kit
缩写：CYR61
Uniprot No.：Q9ES72
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat CYR61 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 87-97 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 96-101 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 96-104 | |||||
回收率：
| The recovery of rat CYR61 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 92-102 | ||||
| EDTA plasma (n=4) | 96 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.6252.5292.5772.41310002.0621.9562.0091.8455001.3841.3661.3751.2112500.8710.8810.8760.7121250.5510.5390.5450.38162.50.3420.3510.3470.18331.250.2490.2550.2520.08800.1640.1630.164 | ||||||
货期：3-5 working days
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