首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠细胞色素P450,家族7亚科A多肽1(CYP7A1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CYP7A1含量。CYP7A1 是胆固醇代谢中的关键靶点。其背景在于它是肝脏胆固醇转化为胆汁酸的限速酶。研究机制方面，主要聚焦于其在调节胆固醇稳态、脂质代谢中的作用，通过调控它的表达和活性，为治疗高胆固醇血症、脂肪肝等疾病提供潜在策略。试剂盒检测范围为93.75 pg/mL-6000 pg/mL，本试剂盒可精准量化其表达水平，为探索胆固醇代谢通路、肝脏生理机制及药物代谢相关研究提供可靠工具。适用于体外实验中对CYP7A1蛋白表达变化的动态监测，如胆汁酸合成研究、代谢性疾病模型分析或药物干预实验中的生物标志物检测，满足基础科研中对靶标分子定量分析的需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cyp7a1 ELISA kit; Cyp7Cytochrome P450 7A1 ELISA kit; 24-hydroxycholesterol 7-alpha-hydroxylase ELISA kit; EC 1.14.14.26 ELISA kit; CYPVII ELISA kit; Cholesterol 7-alpha-hydroxylase ELISA kit; Cholesterol 7-alpha-monooxygenase ELISA kit; EC 1.14.14.23 ELISA kit
缩写：CYP7A1
Uniprot No.：P18125
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：93.75 pg/mL-6000 pg/mL
灵敏度：23.4 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat CYP7A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:1 | Average % | 87 | |
| Range % | 82-92 | ||
| 1:2 | Average % | 96 | |
| Range % | 92-99 | ||
| 1:4 | Average % | 98 | |
| Range % | 93-103 | ||
| 1:8 | Average % | 95 | |
| Range % | 90-98 |
回收率：
| The recovery of rat CYP7A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-97 | ||||
| EDTA plasma (n=4) | 96 | 92-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 60002.4672.4142.4412.349 30002.0111.9992.0051.913 15001.5941.5841.5891.497 7501.0851.0671.0760.984 3750.6830.6720.6780.586 187.50.4010.3870.3940.302 93.750.2350.2210.2280.136 00.0930.0910.092 | ||||||
货期：3-5 working days
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