首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠细胞色素C(CYCS)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CYCS含量。CYCS 即细胞色素 c，是位于线粒体内膜外侧的水溶性蛋白。它在细胞呼吸的电子传递链中发挥关键作用。研究发现，其从线粒体释放到细胞质会激活凋亡相关蛋白，进而启动细胞凋亡机制，在细胞死亡调控等研究领域备受关注。试剂盒检测范围为78 pg/mL-5000 pg/mL，适用于大鼠模型中CYCS相关的基础研究，例如线粒体功能评估、凋亡信号通路探索、药物诱导的细胞损伤研究或心血管疾病模型的分子机制分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CycsCytochrome c ELISA Kit; somatic ELISA Kit
缩写：CYCS
Uniprot No.：P62898
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat CYCS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 87-94 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 85-96 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 90-95 | |||||
| 1:8 | Average % | 91 | ||||
| Range % | 87-96 | |||||
回收率：
| The recovery of rat CYCS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-96 | ||||
| EDTA plasma (n=4) | 90 | 84-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected50002.5812.4582.5202.42725002.2342.1862.2102.11712501.6381.6211.6301.5376251.1031.0971.1001.0073120.6920.6720.6820.5891560.3930.3860.3900.297780.2360.2230.2300.13700.0940.0920.093 | ||||||
货期：3-5 working days
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