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产品描述：人基质细胞衍生因子1β(SDF-1β/SDF1B)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的SDF-1β/SDF1B含量。试剂盒检测范围为125 pg/mL-8000 pg/mL，该产品适用于体外研究SDF - 1β在细胞趋化实验、炎症模型构建、肿瘤微环境分析中的表达变化，也可用于评估药物或基因干预对SDF - 1β信号通路的影响，为免疫调控、癌症转移机制及再生医学等领域提供可靠的工具支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：12-O-tetradecanoylphorbol 13-acetate repressed protein 1 ELISA Kit; AI174028 ELISA Kit; C-X-C motif chemokine 12 ELISA Kit; Chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1) ELISA Kit; Chemokine (C-X-C motif) ligand 12 ELISA Kit; Chemokine CXC motif ligand 12 ELISA Kit; cxcl12 ELISA Kit; hIRH ELISA Kit; hSDF-1 ELISA Kit; Intercrine reduced in hepatomas ELISA Kit; IRH ELISA Kit; OTTHUMP00000019491 ELISA Kit; PBSF ELISA Kit; Pre-B cell growth-stimulating factor ELISA Kit; SCYB12 ELISA Kit; SDF 1 ELISA Kit; SDF-1 ELISA Kit; SDF-1-alpha(3-67) ELISA Kit; SDF-1a ELISA Kit; SDF-1b ELISA Kit; SDF1_HUMAN ELISA Kit; SDF1A ELISA Kit; SDF1B ELISA Kit; Stromal cell-derived factor 1 ELISA Kit; Stromal cell-derived factor 1 delta ELISA Kit; Stromal cell-derived factor 1 gamma ELISA Kit; Stromal cell-derived factor 1a ELISA Kit; Stromal cell-derived factor-1 alpha ELISA Kit; Thymic lymphoma cell-stimulating factor ELISA Kit; Tlsf ELISA Kit; TLSF-a ELISA Kit; TLSF-b ELISA Kit; Tlsfa ELISA Kit; Tlsfb ELISA Kit; TPAR1 ELISA Kit
缩写：SDF-1β/SDF1B
Uniprot No.：P48061
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：125 pg/mL-8000 pg/mL
灵敏度：31.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SDF1B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 80-94 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-103 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 92-105 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 86-99 | |||||
回收率：
| The recovery of human SDF1B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-99 | ||||
| EDTA plasma (n=4) | 98 | 90-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected80002.8492.6642.7572.61440002.4372.2912.3642.22120001.6771.6031.6401.49710000.8410.8120.8270.6845000.4700.4590.4650.3222500.2980.2890.2940.1511250.2270.2220.2250.08200.1440.1410.143 | ||||||
货期：3-5 working days
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